PBMC for APC-based Immunotherapy

Peripheral blood mononuclear cells (PBMCs) are a group of cells isolated from peripheral blood that play a crucial role in the immune system. This cell population includes T cells, B cells, monocytes, and natural killer cells. More importantly, PBMCs contain numerous APC populations such as dendritic cells (DCs), monocytes/macrophages, and B cells, all of which can function as APCs to present antigens and thereby stimulate an immune response. Like the DC vaccine platform, antigen-pulsed PBMCs can serve as a novel vaccine platform.

PBMC-based immunotherapy holds great potential for improving patient outcomes and providing new treatment options for cancer and other diseases. PBMCs isolated from a patient's or healthy donor's blood can be engineered to enhance their ability to recognize and attack tumor cells. This can involve stimulating the PBMCs with specific antigens or genetic modification. Upon the modification of PBMCs, they are infused back into the patient's system to target and eliminate cancer cells.

Fig.1 Schematic overview of PBMC-derived cell therapy.¹

PBMC Platform

PBMCs are a diverse population of immune cells that play a crucial role in the adaptive immune response. As a heterogeneous population consisting primarily of lymphocytes and monocytes, PBMCs play a crucial role in various cellular therapy strategies. Through precise genetic engineering techniques, we can customize PBMCs to exhibit specific characteristics, making them ideal candidates for cell-based therapies.

• Preparing and Isolating PBMCs
  Proper isolation and preparation of PBMCs are essential for obtaining reliable and reproducible results in research and therapeutic applications. Our service offers cutting-edge techniques and expertise in isolating PBMCs from whole blood samples with high efficiency and purity. By utilizing state-of-the-art equipment and protocols, we ensure the isolation process is carried out meticulously to maintain the viability and functionality of the PBMCs.
• Isolating Specific Cell Populations
  Creative Biolabs provides a series of techniques to isolate specific cell populations from PBMCs for further analysis or therapeutic purposes. These methods typically include density gradient centrifugation, magnetic cell sorting, and flow cytometry. For example, monocytes can be isolated and induced to differentiate into APCs, such as dendritic cells, which play a critical role in initiating and regulating immune responses.
• Engineering PBMCs
  Creative Biolabs offers cutting-edge services to engineer PBMCs for the development of advanced cell-based therapies. Through genetic editing and engineering techniques, PBMCs can be tailored to exhibit specific traits, behaviors, or functions that are relevant to the study of various diseases, immune responses, and therapies.
  
  • Antigen-pulsed PBMCs
  • Engineering PBMCs to express chimeric antigen receptors (CARs)
  • Engineering PBMCs to express tumor antigen-specific T cell receptors (TCRs)
  • Engineering PBMCs to express cytokines or regulatory molecules
  • Checkpoint silence-based PBMC engineering

Our Engineering Techniques

Genetic modification of PBMCs involves the introduction of foreign genetic material into these immune cells to induce specific changes in their function or behavior. At Creative Biolabs, our scientists have the ability to genetically modify PBMCs, enabling the introduction of exogenous DNA or RNA into the PBMCs. Our skilled team of experts specializes in manipulating the genetic makeup of PBMCs to enhance their therapeutic potential. We employ various technologies to engineer PBMCs, including viral vectors, gene editing, siRNA delivery, or transfection techniques. Partner with us to access tailored genetic modification strategies that propel your research to new heights in the realm of cell therapy.

Reference

1. Zhou, Yang, et al. "Engineering induced pluripotent stem cells for cancer immunotherapy." Cancers 14.9 (2022): 2266.

For Research Use Only | Not For Clinical Use