In the field of Antibody-Directed Enzyme Prodrug Therapy (ADEPT), Creative Biolabs provides top-class services in the design, synthesis, and modification of enzyme prodrugs. Our team comprises seasoned scientists who use precise molecular design and optimization to assist you in achieving more efficient and safer targeted therapies for tumors.

Introduction

In ADEPT, the design, synthesis, and modification of prodrugs play a crucial role. The design of the prodrug is essential to ensure that it remains inactive in the body and is activated only at the target location. Optimizing the synthesis process can improve the prodrug's purity and stability, thereby reducing the risk of side effects. Additionally, modifying the prodrug can not only enhance its pharmacological properties but also introduce specific functional groups for more precise targeting.

Creative Biolabs enables more precise and effective targeted therapy by designing, synthesizing, and modifying prodrugs. In our labs, we focus on rational molecular design to optimize the pharmacokinetic properties of prodrugs, thereby enhancing their active release within the tumor microenvironment. Meanwhile, our research team selects the appropriate enzymes and prodrug conjugation methods to strengthen targeting capabilities and improve therapeutic outcomes. Furthermore, integrating biocompatible materials or designing controlled release systems facilitates prolonged therapeutic effects. Additionally, we offer a variety of modification strategies for enhancing the pharmacokinetic properties and biocompatibility of the drugs. We are confident that with precise modifications and improvements, your research will achieve even greater success.

Fig.1 Prodrug Activation Mechanism.^1,3

Services

In the realm of drug development, prodrug molecules are gaining increasing attention due to their ability to enhance drug efficacy and reduce side effects through metabolic conversion within the body. Utilizing advanced chemical synthesis techniques, Creative Biolabs is equipped to design and synthesize novel prodrug entities tailored to specific therapeutic needs.

• Targeted Design and Screening Service
  Using computer-aided drug design (CADD) technologies, Creative Biolabs can conduct targeted design of prodrugs based on structural predictions of the target and pharmacological characteristics, ensuring high selectivity towards specific enzymes and enhancing drug activity.
• Synthesis and Optimization Service
  We offer specialized chemical synthesis services to create prodrugs with diverse structures. We enhance their pharmacokinetic characteristics, including solubility and bioavailability, by conducting several rounds of optimization.
• Biotransformation Studies
  Our laboratory conducts systematic investigations of the biotransformation processes of synthesized prodrugs through in vitro and in vivo experiments. We assess their metabolic pathways and release mechanisms to ensure safety and efficacy.
• Pharmacological Evaluation Service
  Our team performs pharmacological assessments in various experimental models (like cell lines) to assist clients in validating the bioactivity and potential therapeutic effects of prodrugs.

Fig.2 Blood Clearance Testing of the ADPET Strategies.^2,3

Process

1. Target Selection and Drug Design: Creative Biolabs will identify suitable targets based on the mechanisms of the tumor and biological targets. By using techniques like computer-aided drug design and high-throughput screening, we aim to establish the initial structure of the compounds. During the design phase, it is essential to consider the drug's pharmacological activity, selectivity, metabolic stability, and pharmacokinetic properties.

2. Development of Synthesis Pathways: Once candidate compounds are identified, our team will begin developing synthesis routes. Employing organic synthesis techniques, we will optimize reaction conditions to achieve the desired compound. Throughout this process, we will ensure high efficiency and reproducibility of the synthesis while also considering environmental friendliness.

3. Modification and Optimization: After synthesizing the initial compounds, we will enhance their activity and bioavailability through structural modification. This could involve introducing substituents, adjusting molecular size, or altering polarity. Specific modifications can improve enzyme recognition and increase conversion efficiency within target cells.

4. In Vitro and In Vivo Evaluation: Our laboratory will perform a series of in vitro experiments on the modified prodrugs, such as cytotoxicity assays and metabolic stability tests. Additionally, we will conduct in vivo pharmacokinetic studies to assess the metabolic conversion and biodistribution within animal models.

Features

ADEPT has proven its significant role in transformative cancer therapy possibilities. Unlike conventional therapies, ADEPT allows for more precise delivery of drugs to cancer cells, effectively minimizing side effects and enhancing treatment efficacy. In this area, Creative Biolabs is dedicated to providing high-quality design, synthesis, and modification services for enzyme prodrugs, supporting your research and development initiatives.

• Efficient Synthesis: Using advanced synthesis techniques, we can rapidly and efficiently produce target enzyme prodrugs. Our strict quality control system ensures that each batch meets the required purity and activity standards for both research and clinical applications.
• Versatile Modifications: We offer a range of chemical modification services aimed at improving the stability, selectivity, and biocompatibility of enzyme prodrugs. Our modifications not only enhance the therapeutic effectiveness but also reduce the impact on normal cells, making treatments safer.

Creative Biolabs offers specialized services in the design, synthesis, and modification of ADEPT enzyme prodrugs. We will help the design of enzyme prodrug molecular structures to meet the specific needs of our clients, taking into account biological characteristics and pharmacokinetics. By chemically modifying the prodrugs, we can enhance their stability and biocompatibility within the body, thereby improving their therapeutic effectiveness. If you are interested in our services, please feel free to contact us at any time.

References

1. Miller, Justin J., et al. "Structure-guided microbial targeting of antistaphylococcal prodrugs." Elife 10 (2021): e66657.
2. Wang, Hao, et al. "A Fusion Protein of rgd4c and β-lactamase has a favorable targeting effect in its use in antibody-directed enzyme prodrug therapy." International journal of molecular sciences 16.5 (2015): 9625-9634.
3. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use