Single Cell Immune Profiling

The development of immune cell therapy often requires an in-depth understanding of their impact on the entire immune cell system. Our expertise includes flow cytometry analysis and intracellular staining of cell surface markers to view major regulatory proteins. Our Flow Cytometry team has developed and validated a single-cell immunoassay solution, which is a comprehensive approach for identifying and analyzing multiple related immune cells, such as CAR-T cells, CAR-NK cells, CAR-MA cells, adjuvant and regulatory T cells, macrophages and natural killer cells. We can simultaneously examine the adaptive immune response and the cellular environment of the immune pool in hundreds to tens of thousands of immune cells in human or mouse tissues on a cell-by-cell basis.

Through our streamlined workflow, you can prepare samples and libraries, perform immunosequencing and software analysis, detect and analyze other cell readings, reveal the diversity of specific recombinant CAR-immune cells, and gain insights into immune cell analysis.

Single Cell Immune Profiling Capabilities

Fig.1 Multiplexing single cell measurement of CAR-T cells in microchamber.¹

Single cell immune profiling provides the ability to classify specific cells based on hundreds to tens of thousands of immune cell populations. The presence of specific cell surface markers, cytokine expression or phosphorylation of key proteins can be used to immunospecifically phenotype specific subpopulations from heterogeneous starting populations.

Cell surface phenotyping based on surface marker staining
Intracellular flow cytometry for intracellular cytokines, phosphoprotein or nuclear factor analysis
Perform up to 16 color flow cytometers
Immune cell sorting features 8 color strobes and 4 channel sorting
Simultaneous measurement of TCR-T and CAR-T/NK/MA cell surface protein expression and 5' gene expression in the same cells
TCR repertoire analysis in single cells

For more details, please feel free to contact us for project quotations and more detailed information.

Single Cell Immune Profiling Capabilities

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