Based on our advanced toxicity assessment platforms and powerful technology, Creative Biolabs provides a complete range portfolio of assays for toxicity assessment including quantification of the amylase. To meet the service you need, our highly skilled scientists are ready to get started on your projects.

Introduction of Amylase

Amylase is a digestive enzyme that chemically catalyzes the hydrolysis process of starch into sugars. There are three main classes of amylase enzymes: α-Amylase, β-Amylase, and γ-Amylase. And α-Amylase is mainly present in the salivary glands and pancreas of humans and some other mammalian animals. The α-Amylase is closely involved in the digestion and absorption of carbohydrates, as shown in Fig.1. Amylase performs the first step in the crucial digestion process by which cells are provided with the principal energy and carbon source.

Fig.1 Carbohydrate Digestion with salivary and pancreatic amylase. (Draper, et al., 2022)

• Diagnostic Functions of Amylase

Amylase in the serum is produced mainly in the salivary glands and pancreas. If there is cell damage or cell inflammation, amylase will leak out from the damaged cells into the bloodstream, leading to an abnormal concentration rise in the serum. Elevated amylase activity is suggestive of salivary glands or pancreatic inflammation, acute hypoxemia, and certain digestive conditions. Therefore, serial measurement of serum or urine amylase, as one most sensitive non-invasive biomarkers, could be used for diagnostic functions.

• Therapeutic Functions of Amylase

Since α-Amylase is the key enzyme involved in the carbohydrate digestion process, its inhibitors have been regarded as new drugs to treat Type 2 diabetes mellitus (T2DM), hyperglycemia, hyperlipemia, and obesity by preventing starch metabolism. In the gastrointestinal tract, the potential mechanisms of α-amylase inhibitors on the prevention of hyperglycemia by whole cereal food intake are shown in Fig.2. Inhibitors of α-Amylase could limit the hydrolysis of starch by blocking the active centers of α-Amylase, thereby delaying intestinal absorption of glucose.

Fig.2 Potential mechanisms of α-amylase inhibitors on the prevention of hyperglycemia. (Gong, et al., 2020)

Quantification of Amylase services at Creative Biolabs

In the pharmaceutical research and diagnosis technique development, toxicity assessment by quantification of amylase, as a non-invasive biomarker, could help detect salivary glands and pancreas tissue damage. It could also help real-timely monitor the circulatory system in various pathological processes.

Considering its importance, amylase detection has been researched well by scientists of Creative Biolabs. The methods of amylase quantification include reducing sugar methods, enzymatic methods, chromogenic methods, Iodine-starch methods, turbidimetric methods, and so on. While each method has its own merits and demerits, our scientists in Creative Biolabs, with decades of in-depth study of this toxicity assessment area, will offer you the most suitable and advanced custom analytic service. The lab of Creative Biolabs is equipped with fully automated multiplex analyzers and analysis software. As one most potent professional technical developers, We will bridge the gap between your sample materials and a well-rounded assessment result of your specific treatment strategies.

Features of Quantification of Amylase Services

• Advanced technical platform with high sensitivity, accuracy, and repeatability
• Fully customized design to meet every specific demand
• Reliable lab report with timely update

Amylase may also be quantified along with other enzymes and compounds described detailedly in our Toxicity Assessment Service. No matter which panel of biomarkers you need to test, our experienced experts at Creative Biolabs will do their best to complete your project. If you are interested in this, please feel free to contact us directly.

References

1. Draper, J.; et al. Human nutrition 1e (University of Hawaii). LibreTexts. 2022: 6780.
2. Gong, L.; et al. Inhibitors of α-amylase and α‐glucosidase: potential linkage for whole cereal foods on prevention of hyperglycemia. Food science & nutrition. 2020, 8(12): 6320-6337.

For Research Use Only | Not For Clinical Use