Being a certified contract research organization (CRO) with GLP facility, Creative Biolabs is experienced in offering one-stop Protein Degraders service. To support institutes and pharmaceutical industry for therapeutic Protein Degraders small molecule drugs, we offer various services to assess the target protein ubiquitination targeted by Protein Degraders candidates.
As a rapidly emerging alternative therapeutic strategy, Protein Degraders has the potential to address many of the current challenges faced in modern drug development approaches. Protein Degraders employs small molecules composed of a target protein ligand, a linker, and an E3 ligase ligand. Following “target protein-Protein Degraders-E3 ligase” ternary complex formation, the exposed lysines on the target protein will be ubiquitinated by E3 ligase complex. Upon poly-ubiquitination of the target protein, it will be recognized by proteasome and degraded into amino acids and small peptides (see Fig.1).
Fig.1 Schematic of the PROteolysis TArgeting Chimera (Protein Degraders) technology. (Neklesa, 2017)
As per the above Protein Degraders principles, we can draw that E3 ligase complex-mediated ubiquitination for target protein is crucial determinant for target protein degradation. To evaluate the target protein ubiquitination efficacy caused by PRORAC candidates, Creative Biolabs has developed a series of different approaches to facilitate your Protein Degraders research. To avoid false positive, we add the controls for each assay, including proteasome or ubiquitination inhibitors. Our approaches are listed below, including but not limited:
MS-based proteomics has become a powerful technique for the analysis of protein ubiquitination. It measures the mass-to-charge ratio (m/z) of ions to identify and quantify ubiquitination in simple and complex mixtures. Creative Biolabs offers MS-based approach to characterize the target protein ubiquitination after treated with Protein Degraderss. We ensure high efficiency and high sensitivity.
WB is a widely used technique in molecular biology to detect specific proteins via corresponding antibodies. Creative Biolabs offers a series of processes - cell lysis, immunoprecipitation, washing, and anti-ubiquitin immunoblotting to evaluate the target protein ubiquitination treated with Protein Degraders. The degree of ubiquitination can be estimated by comparing the ratio of ubiquitinated/unmodified target protein in different experimental conditions.
Fig.2 (A-D) Western blot ubiquitination results (Ottis, 2017); (E) Workflow for enriching ubiquitylated proteins (Heap, 2017).
As the important signal prior to target protein degradation, target protein ubiquitination is a crucial index to indicate the degradation efficacy. Creative Biolabs is here to serve both MS and WB approaches to evaluate the ubiquitination efficacy, and we are committed to choosing the best optimal assay to maximize the success of your projects. Please contact us for any questions or further communication.
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