Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
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HighlightsAcetylation is a form of post translational modification (PTM) of proteins. It can affect the cellular chromosome structure and the activation of transcriptional regulators in the nucleus. It is also involved in the regulation of the cell cycle, metabolism, tumors, and polyglutamine diseases. Therefore, the study of protein acetylation is crucial for disease research and drug development. However, PTM specific antibodies, including acetylation-specific antibodies, are not easily available. Based on technologies such as the hybridoma platform and phage display platform, Creative Biolabs offers comprehensive antibody development services to provide you with customized acetylation-specific antibodies.
Acetylation modification is the process by which a protein transfers and adds an acetyl group to a protein lysine residue or the N-terminal end of a protein, catalyzed by an acetyltransferase (or non-enzyme). There are two main types of acetylation modifications: acetylation modification at the protein N-terminal and acetylation modification at the protein lysine. Most protein N-terminal acetylation modifications occur on eukaryotic proteins and are catalyzed by N-acetyltransferases. Acetylation modification on lysine is a reversible process, mainly catalyzed by lysine acetylases and lysine deacetylases. Acetylated proteins affect physiological processes such as apoptosis, cell metabolism, signal transduction, and protein stability by regulating protein function, chromatin structure, and gene expression. They play an important role in metabolic diseases, metabolic disorders, tumorigenesis, and other pathological processes.
Given the important role of protein acetylation modifications in physiological and pathological processes, studying protein acetylation modifications is essential. It consists of the following main steps: determining the presence of acetylation of target proteins and screening acetylases/deacetylases; identifying acetylation sites; resolving the effects of acetylation on target proteins; and exploring the biological functions of acetylation of target proteins. In this process, acetylation-specific antibodies are crucial.
Acetylated protein antibodies can be used to detect changes in protein acetylation levels, focusing on understanding the active state of the protein. However, the development of acetylated protein antibodies is not an easy task. In general, the development of acetylated protein antibodies consists of five main steps.
The first step is selecting a suitable acetylation site. A biologically meaningful acetylation site is selected based on a review of literature and data, combined with the needs of the study. This site should be able to be catalyzed by a specific enzyme and regulate the activity of the protein.
The next step is designing a suitable peptide antigen. A peptide sequence containing an acetylation site is designed that is long enough to be recognized by the immune system. At the same time, this sequence should be short enough to avoid cross-reactivity with other unrelated proteins.
Then, a suitable animal for immunization is selected. An animal capable of producing an efficient antibody response is chosen for immunization. Typically, rabbits and mice are the most commonly used animals. Depending on the experimental purpose and the response of the animal, the immunization procedure is optimized to obtain the best antibody yield and specificity.
Finally, antibody specificity is verified. The specificity of the antibody is confirmed using a variety of experimental methods to ensure that it specifically recognizes the acetylated form of the target protein.
Creative Biolabs has a wealth of knowledge about antibody discovery. We would be happy to discuss our knowledge and experience in acetyl-specific antibody development with you.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
