Unlike water-soluble proteins, most membrane proteins cannot be solubilized or stabilized in aqueous solutions without the aid of any amphipathic materials. One of the most common difficulties in handling membrane proteins is the choice of amphipathic materials. Amphipathic materials can play an indispensable role in the extraction, solubilization, stabilization, characterization, functionalization, and activation of membrane proteins.
Custom Detergent Screening Service | Custom Lipid Screening Service |
Depending on the membrane protein and the intended use, different detergents may be needed. Research on, development of, and use of membrane proteins have been severely hampered by the possibility of unfolding, conformational changes, loss of function or activity, and/or aggregation of membrane proteins due to incorrect or inadequate detergent use. With the help of our robust detergent screening technology, Creative Biolabs can quickly determine which detergent or detergent combination is best for each membrane protein. | Membrane proteins solubilized or reconstituted in the aforementioned forms can exhibit significant variations in folding, conformation, oligomeric state, function, and activity due to a variety of lipid types. Lipid screening is therefore typically crucial for determining the ideal circumstances for maintaining the solubilized/incorporated membrane proteins in their native-like form. Creative Biolabs offers complete lipid components for screening. |
Cell Membrane based Native Membrane Protein Extraction Service | |
One of the most important processes in the synthesis of membrane proteins is the extraction of membrane proteins from cell membranes. Membrane proteins produced in cell membranes, in contrast to soluble proteins, are typically removed using detergents and relatively mild lysis conditions. Membrane protein misfolding, conformational changes, aggregation, and/or precipitation can be irreversibly caused by improper detergent use or conditions. We at Creative Biolabs are pleased to provide our expert cell membrane protein extraction service. |
Screening for the best detergent or micelle-forming lipid to construct micelles solubilizing a membrane protein can be quite a labor- and capital-intensive task. Each detergent or micelle-forming lipid may have very different stabilizing or destabilizing effects on a membrane protein. Besides, in most cases, different micelles need to be used for membrane protein extraction from different sources, as well as for different applications. With rich experience in this area and a series of detergents and lipids available, Creative Biolabs has built a comprehensive detergent screening platform and lipid screening platform to find the best micelles for our customers' specific membrane protein targets with high purity, stability, and yield. Please feel free to contact us for more information.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
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