Fluorescent Dyes Labeling Services

Creative Biolabs offers a variety of fluorescent dye labeling services for worldwide customers for both research and commercial use. Based on years of conjugation experience, our custom fluorescent antibody and protein labeling service includes experimental design and development of protocols for the preparation of conjugates to be used in various detection systems.

Introduction of Fluorescent Dyes Labeling

Fluorescent dye labeling, often known as fluorescent tag or fluorescent probe, is the use of chemically bound molecules to detect biomolecules such as proteins, antibodies, amino acids, or nucleic acids. Most importantly, the assays can be performed in real-time, in solution, with high-time resolution and sensitivity down to single molecules. Moreover, the advantages of using a fluorescently labeled antibody include harmlessness to target molecule folding and function, brighter signal, multiplexing capabilities, and ease of use (many are available pre-conjugated to many different colors of dyes).

Fig.1 Illustration of methods used for fluorescence labeling.^1,4

Applications of Fluorescent Dyes Labeling

• Bioimaging
  Fluorescent dyes are extensively used for fluorescent labeling of various biomolecules, such as peptides, proteins, antibodies, and nucleic acids, to visualize cellular structures and processes. They make it possible for scientists to investigate cellular mechanisms, protein mobility and localization within cells, protein-protein interactions, gene expression, and the localization of specific molecules within cells.
• Flow cytometry
  Fluorescent dyes are used in flow cytometry to label and analyze cells based on their physical and chemical properties. Different dyes can be used simultaneously to identify and quantify specific cell populations, measure cell viability, and assess cell cycle progression. This strategy is frequently used in medical laboratories to diagnose disorders and assess the efficacy of therapies.
• Immunohistochemistry
  Fluorescent dyes are employed in immunohistochemistry techniques to label specific antigens within tissue sections. This allows for the visualization and localization of proteins or other biomarkers of interest within the tissue, aiding in disease diagnosis, understanding cellular function, and studying tissue development.
• Molecular biology research
  Fluorescent dyes are widely used in molecular biology research to label DNA, RNA, and other molecules. They can be used for techniques such as fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR), and DNA sequencing. These techniques enable the detection and analysis of specific DNA or RNA sequences, gene expression profiling, and genetic mapping.
• Drug discovery and development
  Fluorescent dyes are utilized in drug discovery and development processes to study drug-target interactions, evaluate drug efficacy, and assess cellular uptake and distribution of potential therapeutics. They play a vital role in high-throughput screening assays and drug delivery system development.

Fluorescent Dyes Labeling Services Provided by Creative Biolabs

With extensive experience and professional scientists, Creative Biolabs can conjugate your antibody or protein to numerous fluorescent dyes. To verify that the observed fluorescence is a result of staining rather than an unspecific artifact, our skilled staff will work closely with you to help select the most suited dye and labeling site according to your specific requirements. Starting from customer-supplied antibodies or Creative Biolabs generated custom antibodies, we deliver fluorescent dyes labeled antibodies in a relatively short duration. Furthermore, each labeled service is meticulously monitored by our quality assurance center. The fluorescent dyes we can offer are shown below, which include but are not limited to:

• BODIPY Derivatives
• Rhodamine Derivatives
• Lucifer Yellow Derivatives
• Coumarin Derivatives
• Fluorescein Derivatives
• Enzyme
• Organometallic Compound
• Cascade Blue Derivatives
• Phycobiliprotein Derivatives

Features of Our Fluorescent Dyes Labeling Service

• Wide selection of up to 9 unique fluorescent dyes to support your project
• Rich experience and mature technology to ensure your program a success
• Fast turnaround time with a relatively low price
• Full range of technical support

Creative Biolabs offers an extensive range of fluorescent dye labeling services for global researchers in the most high-quality and cost-effective way. If you are interested in how we can help you with the development of your programs, please feel free to contact us and get more detailed information.

Published Data

1. Fluorescent Probe for Optical Fluorescence Imaging of Native Proteins

Fig.2 Detection of the Fluorescently Labeled NPI.^2,4

This study showcased a highly sensitive fluorescence imaging technique for visualizing a single native protein of interest (NPI), utilizing a eukaryotic translation system that incorporated a cell-permeable fluorescent dye with a free carboxyl group. In living cells, the carboxyl group of the dye interacted with lysine residues on acceptor peptides, and the genetically encoded recognition system achieved nearly 100% fluorescence labeling efficiency. Nickel-nitrilotriacetic acid (Ni-NTA) beads efficiently bound the labeled NPI for detection directly in cells, without the need for purification. The labeling strategy met key criteria for fluorescent NPI measurement using universal carboxyl fluorescent dyes. This approach holds promise for investigating complex biological and ecological processes, enabling single-molecule analysis, and ultra-sensitive NPI detection in nanotechnology applications.

2. Fluorescent Dyes Labeling in Nanoscale Flow Cytometry

Fig.3 Working strategy of the methods described in this study.^3,4

Emerging high-resolution cytometric methods have increased the need for efficient fluorescent labeling techniques to visualize and detect extracellular vesicles (EVs). These labels must be bright enough to detect individual EVs without generating artifacts. This study introduced nanoFACS, a high-resolution flow cytometry method that combined light scattering, fluorescence parameters, and sample enumeration to assess various labels. The approach efficiently stained single EVs, maximizing the fluorescence signal-to-background ratio while preserving functional EV properties. Specifically, researchers compared lipid-, protein-, and RNA-based staining methods and developed this strategy using the amine-reactive fluorescent label 5-(and-6)-Carboxyfluorescein Diacetate Succinimidyl Ester and size exclusion chromatography to remove excess label.

References

1. Martin-Fernandez, Marisa L., and David T. Clarke. "Single molecule fluorescence detection and tracking in mammalian cells: the state-of-the-art and future perspectives." International Journal of Molecular Sciences 13.11 (2012): 14742-14765.
2. Kim, Jung Min, and Young-Mi Kang. "Optical Fluorescence Imaging of Native proteins using a fluorescent probe with a cell-membrane-permeable carboxyl group." International journal of molecular sciences 23.10 (2022): 5841.
3. Morales-Kastresana, Aizea, et al. "Labeling extracellular vesicles for nanoscale flow cytometry." Scientific reports 7.1 (2017): 1878.
4. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only.