Creative Biolabs has always been devoted to assisting our worldwide clients for developing the next generation of antibacterial therapies. Currently, we proudly present our exclusive service to investigate the mechanism of action to support drug discovery process.
Recently, antibiotic resistance has been a global problem for antibacterial drugs. For instance, some antibiotics fail to cross bacterial membrane; some antibiotics fail to cross cell wall, and some antibiotic antibacterial target sites are altered. To improve drugs' efficiency and reduce their toxicity, understanding particular modes of actions is critical. Although several reviews have explained the MOA of antibacterial drugs, not all antibacterial compounds have known mechanisms of action. Creative Biolabs provides several ways of MOA analysis for antifungals, which include the following:
Interference with Folate Biosynthesis
Folate is an essential vitamin that is a critical precursor for the biosynthesis of purines, pyrimidines, and amino acids. Prokaryotic and lower eukaryotic microorganisms synthesize folate in the cells by the support of several enzymes. The importance of folate makes folate biosynthesis pathway a key target for antimicrobial therapy. The folate biosynthesis pathway comprises six committed enzymes: GTPCH, NUDIX pyrophosphatase, DHNA, HPPK, DHPS and DHFS, and they can be potential targets for novel antibiotics.
Interference with Protein Synthesis
Protein synthesis is an essential process for the multiplication and survival of all bacterial cells. They key targets for protein synthesis is ribosome. A large number of clinically useful antibiotics targeting this complex translational ribonucleoprotein machinery have been identified. Creative Biolabs has developed an excellent drug discovery platform to determine the mode of action of antibacterial drugs related with protein synthesis. Generally, antibiotics that target the ribosome almost exclusively bind to one of the three key sites: the decoding (or A-site) on the 30S, the peptidyl transferase center (PTC) on the 50S, and the peptide exit tunnel on the 50S. Creative Biolabs can help you determine the binding sites on ribosome for novel antibiotics.
Interference with Nucleic Acid Synthesis
Nucleic acid directs the synthesis of protein, control the production of enzymes, and are responsible for the transfer of genetic information from one offspring to another. Many antibacterial drugs can inhibit nucleic acid synthesis pathway to kill pathogenic bacteria. The mechanisms of antibiotics that interfere with nucleic acid synthesis (nucleic acid inhibitor) can be divided into two main classes: inhibition of DNA replication and inhibition of RNA transcription. Creative Biolabs has developed an excellent drug discovery platform to determine the mode of action related to nucleic acid synthesis for antibacterial drugs.
Interference with Cell Wall Synthesis
Inhibit cell wall synthesis is one of the most common modes of action for antibiotics. By inhibiting peptidoglycan synthesis, the growth of bacteria is prevented. These bacteria will be subjected to osmotic lysis. Generally, the modes of actions of antibiotics for interference with cell wall include interference with peptidoglycan, interference with teichoic acids (TA), and interference with lipopolysaccharides (LPS).
Creative Biolabs is a trusted partner in identifying the mode of action for novel antibiotics. For more detailed information, please feel free to contact us or directly sent us an inquiry.
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