Creative Biolabs is one of the well-recognized experts who are professional in in vitro pharmacodynamics study. With years of experience, our scientists can offer high-quality anticancer drug pharmacodynamics study services to meet our clients’ demands precisely.
As malignant tumors are life-threatening and the death rate from these diseases is high, it is desirable to provide new, effective anticancer drugs to patients more expeditiously. During the past years, tremendous advances have been achieved in cancer biology and the development of molecular-targeted therapeutics. Particularly, anticancer drugs against various tumor targets, such as PD1 and PD-L1, have been developed for the treatment of multiple tumors. However, the attrition rate of new anticancer drugs in clinical trials is disappointingly high with the appearance of unwanted side effects. The failure of new drugs at the clinical stage is very costly. Thus, the predictive value of preclinical models assumes critical importance in cancer drug development.
As cancer was primarily considered a disease of uncontrolled cell division, regression in tumor size was considered as the main objective endpoint of the efficacy of a compound in preclinical and clinical anticancer drug development for decades, so identification of a cytotoxic or an antiproliferative compound is main idea for finding anticancer drugs. For rapid screening of new anticancer compounds, numerous murine tumor models of rapidly growing cancer were developed. However, large-scale screening using animal systems as practiced in the past is highly unethical, especially in some certain countries such as Europe and India. In addition, as the academic researcher receives fewer funds or none, in vitro cell-based assays are the preferred screening techniques which are economically good enough to establish the anticancer activity of a compound/ drug and reduce the animal utilization experiments.
Fig.1 Schematic representation of assays and techniques available for the detection of cancer cell viability/ proliferation based on cellular enzyme activity, protein levels, DNA synthesis/ DNA content, ATP levels and membrane integrity. (Ediriweera, 2019)
Generally, evaluation of in vitro anticancer activity is based on the concept of ‘basal’ cytotoxicity of the compounds / drug which affects the basic functions of cells which in turn leads to cellular damage. Changes in metabolic activities such as risings or declines of mitochondrial activity and Adenosine Triphosphate (ATP) levels are the early indications of cellular damage. In addition, damage to cell membrane integrity increases the concentration of enzymes such as Lactate Dehydrogenase (LDH), and fluorescent dye would be taken after DNA fragmentation. Therefore, changes of certain enzymes and substances based on the above-mentioned facts are some of the basic parameters for the estimation of cellular damage.
To date, a panel of sixty different human tumor cell lines from nine different types of cancer (leukemia, nonsmall cell lung cancer, colon cancer, brain cancer, melanoma, ovarian cancer, renal cancer, prostate cancer, and breast cancer) are applied in in vitro pharmacodynamics study of anticancer drugs. Aided by a top technology platform and experienced scientists, we are able to offer a full package of cell-based pharmacodynamics studies for global customers, including but not limited to:
Cell-based in vitro tests are commonly used to evaluate the efficacy of anticancer drugs. In addition, 3D cell culture and patient derived organoid can simulate the in vivo physiological environment and tumor microenvironment more accurately, so they are gaining more and more attention and welcome in in vitro pharmacodynamics study of anticancer drugs.
Creative Biolabs is dedicated to offering in vitro pharmacodynamics study services for anticancer drug development. In addition to in vitro pharmacodynamics studies, we also provide safety pharmacology core battery service, supplemental safety pharmacology studies, and special toxicity study. Our professional scientists are happy to share their expertise with worldwide researchers. If you are interested in the services we provide, please feel free to contact us for more information and get a quote.
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