Fracture Models

Creative Biolabs provides a most common rodent model (either rat or mouse) of bone repair, which is a closed, simple transverse fracture model generated by externally applied blunt trauma. It has been widely applied to assess the safety and efficacy of systemic pharmaceuticals that might affect fracture healing.

Fig. 1 Construction of rat closed fracture models.¹

Induction of Closed Fracture Model

To be brief, after the animals are anesthetized, pin insertion is carried out prior to fracture by making an anterior longitudinal midline incision centered over the knee joint and the pin is inserted into an entry hole in the center of the groove on either the femoral or tibia condyle. Afterward, the fracture is then generated by dropping a weight onto the operated extremity.

Advantages and Applications

• It is closest to the anatomical site, etiology, and fixation method to common long bone fractures seen in the clinical setting.
• Such fractures are typically closed injuries resulting from a traumatic event such as falls and other accidents.
• It is suitable for high-throughput screening due to its simplicity, speed, and reproducibility of the procedure.
• In addition to assessment of potential therapies of bone healing, this model can be used for studies evaluating basic molecular processes that affect endochondral bone formation.
• Moreover, it can be extrapolated to embryonic development and post-natal epiphyseal growth of long bones.

Assessments

To assess the efficacy of potential therapies on fracture healing study, Creative Biolabs provides assessments including but not limited to:

• Biomechanical testing
• BMD and BMC
• Physiological bone turnover markers (blood, urine)
• Ash chemical analysis
• Histomorphometry
• Histology

Additionally, other examples of rodent musculoskeletal disease models that you may be interested include:

• Monosodium Iodoacetate (MIA)-Induced Osteoarthritis Model
• Surgically-Induced Osteoarthritis (OA) Models
• Bone Defect Models
• Orchiectomy-Induced Osteoporosis Model
• Ovariectomy (OVX)-Induced Osteoporosis in Rats
• Adjuvant-Induced Arthritis (AIA) Rodent Model
• Collagen-Induced Arthritis (CIA) Rodent Model
• Collagen Antibody-Induced Arthritis (CAIA) Model

Creative Biolabs provides highly translatable in vivo models and key technologies for preclinical testing of your novel agents. We are also dedicated to the development of new animal models together with the sponsors. If you are interested in any of these models listed above or if you would like to propose a new model or customize an existing model to meet your particular research needs, please feel free to contact us to experience the value of our expert services.

Reference

1. Prodinger, Peter Michael, et al. "Does anticoagulant medication alter fracture-healing? A morphological and biomechanical evaluation of the possible effects of rivaroxaban and enoxaparin using a rat closed fracture model." PLoS One 11.7 (2016): e0159669. doi: 10.1371/journal.pone.0159669. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only.