Creative Biolabs is incorporated with a single mission in mind: empower scientific discoveries by providing access to the world's most advanced lineage tracing tools and services. Its dedicated team of experts and advanced barcode-based lineage tracing technology enable researchers, scientists, biologists, and drug developers to push the limits of the life sciences. Our scientific capabilities cover all aspects of tumor lineage tracing and support the full spectrum of human oncology imaging work.

Introduction to DNA/Cell Barcoding Services

Recently, Creative Biolabs provided an automated DNA/cell barcode-based lineage tracing platform for tumor cell research. We offer a full range of labels for tumor lineage tracing, from hand-made dye labels, fluorescent proteins, and multi-color labeling systems, to DNA/cell barcodes, which customers can choose from according to their needs. Among them, DNA barcoding, a large number of DNA fragments formed by sequence changes, has been proposed as a novel potential tool for labeling individual tumor cells. For example, the DNA barcode of the retrovirus library can provide high-efficiency and high-resolution markers to track blood-associated tumor hematopoietic stem cells and their cell fate.

Moreover, we have also developed new polylox barcodes and CRISPR barcodes that can be used to label individual progenitor cells in vivo. Our CRISPR-based genome edit-induced InDels and mutations can record more mitosis due to their faster mutation rate, playing an important role in establishing finer tumor cell lineage trees.

Up to now, genetic markers for lineage tracing can be broadly categorized into two groups: endogenous markers and exogenous markers. The endogenous markers are usually derived from nuclear genome variations and mitochondrial genome mutations. nuclear genome-based markers often necessitate costly deep sequencing methods like whole-genome or single-cell exome sequencing, thereby limiting their applicability. Consequently, we have also explored mitochondrial mutation markers. Due to their higher mutation rate, high copy number, high heterogeneity, and low cost, they have a broader prospect for tumor cell tracing.

Fig.1 Barcoded-Based Single-Cell Lineage Tracing.¹

In addition, as a leading CRO company focused on tumor lineage tracing solutions, Creative Biolabs enables scientists in our labs to apply the newest cell barcoding screening approaches in tumor progression, pathogenicity, and drug resistance studies. We offer two main types of lentivirus barcode systems for cell barcoding:

• NGS Barcode System

A deep sequencing method based on NGS has a sensitivity of ≤ 1%. It can be used to identify human and mouse tumor cell lines. This system only needs a small amount of samples, and can quickly obtain relevant information at a low cost.

• RNA-Seq Barcode System

mRNA is barcoded during a reverse transcription reaction subsequently incorporated into tumor cells and further processed for sequencing analysis.

Barcode-based Lineage Tracing Technology Services

In the past ten years, Creative Biolabs has proactively adapted to the ever-changing oncology imaging market with our advanced barcode-based lineage tracing technology to support customers' needs. This enables the most versatile portfolio of barcode-based lineage tracing panels available today, including but not limited to:

• Whole-organism combinatorial and cumulative genome editing lineage tracing system
• CRISPR/Cas9 and Cre-loxp barcode lineage tracing system
• Sleeping Beauty transposon barcode lineage tracing system
• Mitochondrial DNA mutations (natural barcodes) lineage tracing system

In our labs, we use a whole-organism combinatorial and cumulative genome editing lineage tracing system for the gradual introduction and accumulation of multiple mutations in DNA barcodes. This multicellular lineage tracking system can be used for large-scale atlas analysis of tumor cells. CRISPR/Cas9 and Cre-loxp barcode systems have been genetically engineered mouse models of sarcoma. Our Sleeping Beauty Tn3 transposon carries a transcriptional STOP cassette that has been generated and widely used as secondary label reporters of transposable enzyme activity in several tumor mouse models. mtDNA mutations have been regarded as personal natural barcodes of single-cell analysis workflows to enable lineage tracing in murine leukemic models.

Fig. 2 In Vivo Lineage Tracing Validates The Contribution of Intermediate Epiblast Region to Neural Crest Lineage.²

Advantages

• The automatic lineage tracing analysis platform
• The fast, accurate results
• The high-cost performance
• The high resolution
• The high specificity

Creative Biolabs's staff is highly experienced in the tumor lineage tracing market and our customers are at the center of all our efforts. We aim to become the most trusted provider of lineage tracing solutions that empower scientific discoveries by providing highly accurate and cost-effective barcode-based services and products for researchers around the world. Founded in 2006 by a group of passionate biologists with decades of oncology research experience and a proven track record of delivering cost-effective tumor imaging solutions. If you are interested in our services, please do not hesitate to contact us for more details.

References

1. Chen, C.; et al. Connecting past and present: single-cell lineage tracing. Protein & Cell, 2022, 13(11): 790-807.
2. Prasad, M. S.; et al. Blastula stage specification of avian neural crest. Developmental biology, 2020, 458(1): 64-74.

For Research Use Only | Not For Clinical Use