Creative Biolabs is a forward-looking CRO company as well as a leading custom service provider in the field of oncology imaging research. With our extensive experience and advanced platform, we have won a good reputation among our worldwide customers for accomplishing numerous challenging projects in tumor lineage tracing services. We guarantee the finest results for our customers all over the world.

Introduction to In Vivo Tumor Lineage Tracing

The mechanisms of genetic changes that lead to aggressive subpopulations in each lineage have been key to uncovering tumor progression and identifying therapeutic targets. So far, tumor lineage tracking technology has become a powerful tool for tracking new cell subpopulations and monitoring the proliferation and migration process within new cell subpopulations.

Therefore, Creative Biolabs has developed a variety of in vivo tumor lineage tracing methods in the context of cancer research. We divide it into two main categories: (a) using site-specific recombinant enzyme systems as markers that can be used to reconstruct tumor lineages. (b) using reports or barcodes in model organisms to enable one to track all the offspring of clones tagged at the start of the experiment. Currently, we have developed a series of new, high-resolution lineage tracing techniques that enable single-cell readout and high-resolution analysis of tumor evolution. At the same time, we are in pursuit of potential in technology innovation, aiming to further increase the scope of the cancer lineage tracing studies, dimension, and precision.

Fig.1 Overview of Modern Lineage Tracing Methods.¹

Services

• Gene-targeting Lineage Tracing Service
  In Creative Biolabs, the introduction of lineage-reporting elements into tumor cells using different recombinases has become a common approach for lineage tracing analysis. Recombinant enzymes, such as Cre-loxP, FLP-FRT, or dre-rox, induce recombination between DNA target sites to promote the expression of specific heritable reporter genes (such as fluorescent proteins) to label specific cell types.
• Barcode-based Lineage Tracing Platform
  Using next-generation sequencing technology and high-diversity DNA barcoding technology, we can achieve a high-resolution quantitative analysis of tumor progression and clonal dynamics. In addition, due to the heritable nature of barcodes, we have successfully mapped the primary tumor-metastasis relationship and identified the origin of drug resistance in tumors.
• Tumor Single Cell Lineage Tracing Technology Services
  Recently, by combining single-cell technology and DNA barcoding, we have designed a novel lineage-tracking technique that pairs DNA barcodes with RNA reporters, producing a cell containing multiple mRNA copies of the DNA barcode. The lineage data can be detected by using scRNA-seq technology. Besides, by developing new barcode types, we can offer a range of epigenomic or proteomic analyses in tumor cells.
• Tumor Lineage Tracing Mouse Model Construction Solutions
  Genetic lineage tracing is the most commonly used and effective way to study the origin and fate of specific tumor cell types. The construction of genetically engineered mouse models is an important part of lineage tracing technology. In Creative Biolabs, we transferred specific transfected red fluorescent protein genes into malignant glioma cells and implanted them into 4-6-week-old nude mice expressing green fluorescent protein (GFP). After 2 to 3 weeks of tumorigenesis, the transplanted tumor tissue was pressed into a slice, and then the invasion and angiogenesis of brain tumors were observed under fluorescence and confocal laser microscopy.

Fig.2 Modeling Barcoding Mutagenesis and Inferring Time-scaled Phylogenies From Lineage Barcodes.²

Highlights

• One-stop tumor lineage tracing solution
• Mature DNA barcode discovery platform
• The high-cost performance
• The rapid and accurate detection system

Human oncology imaging has broad prospects in cancer diagnosis research and medical applications. They can be used for animal modeling, therapeutic effect evaluation, as well as personalized medicine. As a company specializing in biotechnology services, Creative Biolabs provides In vivo tumor lineage tracing to meet the research needs of our clients. If you are interested in our services, please contact us or directly send us an inquiry.

References

1. Wu, S. H. S.; et al. Lineage tracing: computational reconstruction goes beyond the limit of imaging. Molecules and cells. 2019, 42(2), 104.
2. Fang, W.; et al. Quantitative fate mapping: A general framework for analyzing progenitor state dynamics via retrospective lineage barcoding. Cell. 2022, 185(24): 4604-4620.

For Research Use Only | Not For Clinical Use