Cav1.2 Channel

The Cav1.2 calcium channel, also known as the L-type calcium channel, is a voltage-gated calcium channel that plays a crucial role in regulating calcium influx into excitable cells, such as cardiac and smooth muscle cells, as well as neurons. Pharmacological modulation of Cav1.2 channels is an important therapeutic target for the treatment of cardiovascular and neurological disorders. In drug development, assessing the cardiac safety profile of a potential drug is crucial to ensure that it does not cause any harmful effects on the heart, such as arrhythmias or other cardiac abnormalities. By evaluating the drug's impact on the Cav1.2 calcium channel, researchers can better understand its potential effects on cardiac function and assess its cardiac safety profile.

Cav1.2 Channel Assay Services at Creative Biolabs

In drug development, it is important to test compounds for their potential interactions with ion channels like Cav1.2 to assess their effects on cellular function and potential toxicity. Creative Biolabs offers a Cav1.2 calcium channel assay that can be used in toxicology assessment to evaluate whether the test compounds have any adverse effects on Cav1.2 channel function. We have successfully generated various stable ion channel cell lines including HEK 293-Cav 1.2 cell line. Our experts assess test compounds for interactions with the Cav1.2 channel through automated /manual patch clamp assays on the HEK 293-Cav 1.2 cell line. We analyze the data using appropriate statistical methods and curve-fitting techniques to quantify the effects of the tested compounds. The assay provides valuable insights into the evaluation of whether the compounds inhibit or potentiate the Cav1.2 channel.

Benefits of Working with Us

• Provide valuable insights for toxicology assessment and drug development
• Offer customized assays and analysis services for calcium channels
• Screen and evaluate compounds for interactions with calcium channels
• Assess the effects of compounds on calcium channel function
• Characterizing the modulation of calcium channels by different compounds

Work With Creative Biolabs

Creative Biolabs offers in vitro ADME services for drug development and toxicology assessments. Our rigorous ADME testing protocols ensure accurate assessment of compound efficacy and safety, providing valuable insights for optimizing drug candidates for further preclinical and clinical studies. Our scientists have rich expertise and experience in ion channel assays to evaluate the interactions of compounds with calcium channels, including the Cav1.2 channel. Contact us to discuss your Cav1.2 channel projects with our team.

Support Data

Data 1: The effect of calciseptine on the Cav1.2 channel was analyzed by recording calcium currents after exposure to calciseptine 100 nM and 300 nM. The calciseptine exhibited an inhibitory effect on calcium currents in HEK 293-Cav1.2 in a dose-dependent manner.

Host cell: HEK 293-Cav1.2 cell line

Detection method: manual patch clamp

Fig.1 Recording of Cav1.2-mediated calcium currents.^1,2

References

1. Mesirca, Pietro, et al. "Selective blockade of Cav1. 2 (α1C) versus Cav1. 3 (α1D) L-type calcium channels by the black mamba toxin calciseptine." Nature Communications 15.1 (2024): 54.
2. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use