Based on our advanced Chemokine Production Measurement Service and powerful technology, Creative Biolabs has developed an unparalleled platform for assays of production measurement of CCL, to meet our customers' different purposes. Our highly skilled scientists are ready to get started on your projects.

Introduction and Function of Chemokine CCL

CCL chemokines, with an N-terminal CC domain, are a family of more than 20 chemotactic cytokines. They are an important component for communication between cells and tissues, which is crucial for the functioning of the homeostasis of the immune system and tumor development.

The physiological function of CCL chemokines, produced by tumor cells and tumor-associated cells, includes the recruitment of various cells, such as regulatory T cells (Treg), tumor-associated macrophages (TAM), myeloid-derived suppressor cells (MDSC), tumor-infiltrating lymphocytes (TIL) and other immune cells. As shown in Fig.1, CCL chemokines promote the proliferation, migration, and invasion of cancer cells. At the same time, they also play a key role in the angiogenesis and lymphangiogenesis processes, which are essential for organ-specific metastasis of tumor cells. Various CCL chemokines have been reported to be associated with drug resistance in cancer immunotherapies, including the Engineered Cancer Cells with the self-destruction strategy.

Fig.1 The role of CCL in cancer. (Korbecki, et al., 2020)

Chemokine CCL Production Measurement Services at Creative Biolabs

With industry-leading expertise and state-of-the-art single-use equipment, Creative Biolabs has pioneered the measurement of Chemokine CCL Production. The production of IFNs could be measured at Creative Biolabs by various methods including flow cytometry (FC), enzyme-linked immunoabsorbent assay (ELISA), real-time PCR, and so on.

• Measure Chemokine CCL Production by FC

The FC assay allows the measurement of chemokine CCL production by defined cell populations. In the flow cytometry experiment, after cell stimulation, regents are added to prevent chemokine secretion from the cells. Then cells will be fixed, permeabilized, intracellular stained with antibodies against chemokine CCL, and finally detected by Flow Cytometer. The lab of Creative Biolabs is equipped with a Flow cytometer which has multiple lasers and fluorescent channels. Our experienced experts will do cell sorting or cell analysis needed with limited cell subpopulations for the measurement of chemokine CCL production.

• Chemokine CCL Production Measurement by ELISA

ELISA can be used to measure the production of CCL chemokines that are secreted into the supernatant by stimulated cells. ELISA is more sensitive than FC, while could not identify the cells producing chemokine. As for the ELISA procedure, 96-well plates will be incubated overnight with the capture Antibody. The next day, block the wells' surface sufficiently to prevent non-specific bindings, then add the sample or chemokine standards, and finally, the labeled secondary antibody will detect the existence of chemokine. Between these steps, we need to wash plates to remove the background. In the end, we read the absorbance on a spectrofluorometer. The lab of Creative Biolabs is equipped with an automated full-wavelength spectrofluorometer. Our experienced experts will do ELISA for the chemokine production measurement service you need.

• Chemokine CCL Production Measurement by QPCR

QPCR assay has been developed for immunological investigations of the gene expression of human chemokines in infected or stimulated cells. QPCR could help us understand the origin and functions of chemokine-producing and assess the immune system and identify ideal disease biomarkers. As for the QPCR procedure, samples will be collected to extract RNA for the following reverse transcription. QPCR will run with specific primers we designed for interested chemokine genes. The lab of Creative Biolabs possesses real-time qPCR equipment to achieve the optimization and validation of the experiment parameters to have valuable qPCR results. QPCR is a routine project for our experienced experts, and they will do this measurement of IL production with veritable results.

Based on advanced facilities, capabilities, and expertise in chemokine science, Creative Biolabs is confident in solving the problems associated with chemokine. Our comprehensive services include chemokine detection and even related synthetic gene circuits for cancer immunotherapy projects. If you are interested in our services, please feel free to contact us directly.

Reference

1. Korbecki, J.; et al. CC chemokines in a tumor: A review of pro-cancer and anti-cancer properties of the ligands of receptors CCR1, CCR2, CCR3, and CCR4. Int. J. Mol. Sci. 2020, 21: 8412.

For Research Use Only | Not For Clinical Use