HCC1937 was isolated from primary carcinoma of a germ-line BRCA1 mutation carrier. Creative Biolabs offers state-of-the-art services for engineering the breast cancer cell HCC1937 for self-destruction that meet your specific tumor research needs. Besides, we also offer immune reactivity assessment services and toxicity assessment services after engineering.

Breast Cancer Cell HCC1937

Predisposition to breast cancer may arise from germline mutations in either of two tumor suppressor genes, BRCA1 and BRCA2. Mouse embryos that lack wild-type BRCA1 or 2 functions undergo early embryonic arrest which is most likely associated with the activation of cell cycle checkpoints. Besides, primary cells from such embryos reveal spontaneous chromosomal anomalies in the absence of extrinsic DNA damage. Furthermore, murine BRCA1-induced tumorigenesis is promoted by the preexisting inactivation of p53.

HCC1937, the BRCA1-mutated breast cancer cell line, synthesizes a truncated BRCA1 protein that is the product of a disease-producing mutant allele (5382insC). Grade 3BRCA1 analysis revealed that the HCC1937 cell line is homozygous for the BRCA1 5382C mutation. HCC1937 cell line is positive for the epithelial cell-specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19, and negative for expression of estrogen receptor (ER) and progesterone receptor (PR).

Fig.1 HCC1937 cells. (Christophe, et al., 2018)

Engineering Breast Cancer Cell HCC1937 for Self-destruction

Creative Biolabs provides a service that exploits and manipulates the HCC1937 cell line to promote anti-breast cancer immunity. Self-destruction means the strategy which transfects overexpressed cytokines into cancer cells by different technologies, thereby achieving the purpose of engineering HCC1937 cells. This method does not directly stimulate the HCC1937 cells within the tumor bed, thereby avoiding the risk of increasing HCC1937 cell-dependent metastases. Besides, Creative Biolabs also offers Immune Reactivity Assessment Services and Toxicity Assessment Services after engineering.

Fig.2 The workflow of engineering breast cancer cell HCC1937 for self-destruction. (Creative Biolabs)

Our Services

Expert in cancer immunity, Creative Biolabs provides comprehensive services to help you. You can find the exact strategies and assays in the following units:

For more breast cancer cell lines for self-destruction, please visit the unit of Engineering Cancer Cells for Self-destruction.

With a team of scientists who come from different research backgrounds, Creative Biolabs guides you all the way from project inception to high-quality results. If you have interest, please do not hesitate to contact us.

References

1. 1.Scully, R.; et al. Genetic analysis of BRCA1 function in a defined tumor cell line. Mol Cell. 1999, 4(6): 1093-9.
2. Christophe, M.; et al. Cytotoxicity study of gold nanoparticles on the basal-like triple-negative HCC-1937 breast cancer cell line. Journal of Biomaterials and Nanobiotechnology. 2018, 9, 13-25.

For Research Use Only | Not For Clinical Use