GES-1 cells are an immortalized human gastric epithelial cell line. Creative Biolabs offers state-of-the-art services for engineering the gastric cancer cell GES-1 for self-destruction that meet your specific tumor research needs. Besides, we also offer immune reactivity assessment services and toxicity assessment services after engineering.

Gastric Cancer Cell GES-1

Gastric cancer is the fourth leading type of cancer worldwide. In particular, gastric cancer has a high incidence. The abnormal expression of a number of miRNAs, including miR-27a and miR-23a, has already been identified in gastric cancer. These miRNAs may function as oncogenes, based on their upregulated expression in gastric cancer.

Some research clarified the role of miR-23a in gastric cancer, and gene recombination technology was used to construct a eukaryotic expression vector, pcDNA3/pri-23a, for miR-23a. This vector was transfected into the human gastric epithelial GES-1 cell line followed by continuous clonal selection with G418 (Geneticin). The expression of miR-23a was assessed by reverse transcription-semi-quantitative polymerase chain reaction (RT-sqPCR) until a human gastric epithelial monoclonal cell line was obtained, and this cell line stably expressed miR-23a. Furthermore, the expression of miR-23a in gastric adenocarcinoma and normal gastric tissues was considered, and whether miR-23a promotes the growth and invasion of the human gastric epithelium GES-1 cell line was assessed.

Fig.1 Effects of miR-23a on the invasive ability of GES-1 cells. (Chen, et al., 2015)

Engineering Gastric Cancer Cell GES-1 for Self-destruction

Creative Biolabs provides a complete set of services that exploit and manipulate GES-1 cell lines to promote anti-gastric cancer immunity. Self-destruction means the strategy which transfects overexpressed cytokines into cancer cells by different technologies, thereby achieving the purpose of engineering GES-1 cells. This method does not directly stimulate the GES-1 cell line, thereby avoiding the risk of increasing GES-1 cell-dependent metastases. Besides, Creative Biolabs also offers Immune Reactivity Assessment services and Toxicity Assessment services after engineering.

Fig.2 The workflow of engineering gastric cancer cell GES-1 for self-destruction. (Creative Biolabs)

Our Services

Expert in cell therapy and cancer immunity, Creative Biolabs provides comprehensive self-destruction services to help you complete your research. You can find the exact strategies and assays in the following units:

For more gastric cancer cell lines for self-destruction, please visit the unit of Engineering Cancer Cells for Self-destruction.

With a team of scientists who come from different research backgrounds, Creative Biolabs guides you all the way from project inception to high-quality results. If you have interest, please do not hesitate to contact us.

References

1. Chen, L.; et al. Establishment and characterization of a GES-1 human gastric epithelial cell line stably expressing miR-23a. Oncology letters. 2018, 16(1), 977–983.

For Research Use Only | Not For Clinical Use