The gastric cancer cell MKN, a human gastric adenocarcinoma, is established from the poorly differentiated adenocarcinoma of the stomach (medullary type) of a 62-year-old woman. Creative Biolabs offers state-of-the-art services for engineering the gastric cancer cell MKN for self-destruction that meet your specific tumor research needs. Besides, we also offer immune reactivity assessment services and toxicity assessment services after engineering.

Gastric Cancer Cell MKN

Gastric cancer is a common cause of cancer-related deaths worldwide and is often diagnosed at an advanced stage. Gastric cancer is the third primary cause of cancer mortality in the world. The MKN-45 cell line was established in Japan from a poorly differentiated adenocarcinoma of a 62-year-old woman. The MKN-45 cell line expresses wild-type p53 protein and is characterized by amplification of c-met oncogene and promoter mutation of E-cadherin.

• Characteristics of gastric cancer cell MKN

✔ MKN-45 xenograft model is resistant to treatment with BEZ235 in vivo.

✔ Identifying CSCs, which possess properties that contribute to tumor formation, metastasis and recurrence, in gastric cancer models is considered important for developing stem cell-targeting therapies.

✔ The MKN-45 cell line is used to create the CDX model of gastric cancer.

✔ The MKN-45 xenograft model is also resistant to TGF-B growth inhibition, carboplatin, docetaxel and irinotecan.

Fig.1 Morphology of MKN-45 cells. (Bonelli, et al., 2011)

Engineering Gastric Cancer Cell MKN for Self-destruction

Creative Biolabs provides a complete set of services that exploit and manipulate MKN cell lines to promote anti-gastric cancer immunity. Self-destruction means the strategy which transfects overexpressed cytokines into cancer cells by different technologies, thereby achieving the purpose of engineering MKN cells. This method does not directly stimulate the MKN cell line, thereby avoiding the risk of increasing MKN cell-dependent metastases. Besides, Creative Biolabs also offers Immune Reactivity Assessment services and Toxicity Assessment services after engineering.

Fig.2 The workflow of engineering gastric cancer cell MKN for self-destruction. (Creative Biolabs)

Our Services

Expert in cell therapy and cancer immunity, Creative Biolabs provides comprehensive self-destruction services to help you complete your research. You can find the exact strategies and assays in the following units:

For more gastric cancer cell lines for self-destruction, please visit the unit of Engineering Cancer Cells for Self-destruction.

With a team of scientists who come from different research backgrounds, Creative Biolabs guides you all the way from project inception to high-quality results. If you have interest, please do not hesitate to contact us.

Reference

1. Bonelli, P.; et al. Changes in the gene expression profile of gastric cancer cells in response to ibuprofen: a gene pathway analysis. The pharmacogenomics journal. 2011, 11(6): 412–428.

For Research Use Only | Not For Clinical Use