A549 is a common human non-small cell lung cancer cell line, known to be KRAS mutant and EGFR wild type. It is one of the most commonly used cell lines for a wide range of research applications. Creative Biolabs offers state-of-the-art services for engineering the lung cancer cell A549 for self-destruction that meet your specific tumor research needs. Besides, we also offer immune reactivity assessment services and toxicity assessment services after engineering.

Lung Cancer Cell A549

A549 is the most commonly used human non-small cell lung cancer (NSCLC) cell line for basic research and drug discovery. It consists of hypotriploid alveolar basal epithelial cells, which were first developed by removing and culturing pulmonary carcinoma tissue from the explanted tumor of a 58-year-old Caucasian male.

• Characteristics of lung cancer cell A549

✔ Grow as a monolayer, adhering to the culture flask.

✔ Responsible for the diffusion of water and electrolytes across the alveoli.

✔ Ability to synthesize lecithin and contain a high percentage of unsaturated fatty acids.

Fig.1 A549 cells under DIC microscopy. (Wikipedia)

Applications of Lung Cancer Cell A549

The A549 lung cancer cell has been tested and approved by the FDA and finds use in a variety of applications, including in manufacturing constructs for use in clinical trials.

Table.1 Applications of lung cancer cell A549.

Adenovirus Production	Replicating adenovirus constructs that do not need complementation by the viral oncogene, early region 1A (EA1). As a negative control in assays to measure the replication of adenoviruses that lack E1A.
Disease Modeling	Model the alveolar Type II pulmonary epithelium. Model other infections related to the lungs like allergies, asthma, and respiratory infections. Investigate the role of chemokines during the initial local response to infection with Mycobacterium tuberculosis in the human lung.
Drug Development	In vitro as well as in vivo testing of novel drugs.
Enzymatic Study	Test the ability of 2-(2,4-dihydroxyphenyl) thieno-1,3-thiazin-4-one (BChTT) in the inhibition of cancer cell proliferation and study the effects of insulin and insulin-like growth factor 1 on the apoptosis and proliferation of the A549 cells.
CRISPR Delivery	Constructed Cas9 ribonucleoprotein complexes and demonstrated the transfection of Cas9 mediated by receptors into A549 cells via binding to the epithelial growth factor receptor.

Engineering Lung Cancer Cell A549 for Self-destruction

Creative Biolabs provides full-scale self-destruction services which exploit and manipulate lung cancer cell A549 to promote anti-lung cancer immunity. The self-destruction method does not directly stimulate the A549 cells, thereby avoiding the risk of increasing A549 cell-dependent metastases. Self-destruction means the strategy which transfects overexpressed cytokines into cancer cells by different technologies, thereby achieving the purpose of engineering A549 cells. Lastly, Creative Biolabs also offers Immune Reactivity Assessment services and Toxicity Assessment services after engineering.

Fig.2 The workflow of engineering lung cancer cell A549 for self-destruction. (Creative Biolabs)

You can find the exact strategies and assays in the following units:

With a team of scientists who come from different research backgrounds, Creative Biolabs guides you all the way from project inception to high-quality results. If you have interest, please do not hesitate to contact us.

For Research Use Only | Not For Clinical Use