H358 is a human non-small cell lung cancer (NSCLC) cell line which was isolated from the bronchiole of a male patient with bronchioalveolar carcinoma, and can be used in cancer research. KRAS mutations are found in >25% of lung adenocarcinomas. Creative Biolabs offers state-of-the-art services for engineering the lung cancer cell H358 for self-destruction that meet your specific tumor research needs. Besides, we also offer immune reactivity assessment services and toxicity assessment services after engineering.

Lung Cancer Cell H358

Lung cancer is the leading cause of cancer-associated mortality worldwide. Effective therapeutic strategies for lung cancer are extremely limited exemplifying the need for early diagnosis and novel therapeutic interventions. H358 cells were isolated from primary bronchioalveolar carcinoma of the lung from a Caucasian male taken before treatment.

• Characteristics of lung cancer cell H358

✔ The presence of granules characteristic of Clara cells.

✔ Do not express UDP-glucuronosyltransferases, but do express glutathione-S-transferase and phenol sulphotransferase.

✔ Expression of SP-A protein and RNA, the major surfactant-associated protein.

✔ A lack of p53 protein.

✔ Tumourigenic in athymic nude mice, and exhibit a doubling time of 38 hours in RPMI 1640 medium.

✔ A tool for analyzing the role of glucuronic acid conjugation in the inactivation of chemicals in intact cells.

Fig.1 H358 human NSCLC cells. (Lennon, et al., 2014)

Engineering Lung Cancer Cell H358 for Self-destruction

Creative Biolabs provides full-scale self-destruction services which exploit and manipulate lung cancer cell H358 to promote anti-lung cancer immunity. The self-destruction method does not directly stimulate the H358 cells, thereby avoiding the risk of increasing H358 cell-dependent metastases. Self-destruction means the strategy which transfects overexpressed cytokines into cancer cells by different technologies, thereby achieving the purpose of engineering H358 cells. Besides, Creative Biolabs also offers Immune Reactivity Assessment services and Toxicity Assessment services after engineering.

Fig.2 The workflow of engineering lung cancer cell H358 for self-destruction. (Creative Biolabs)

You can find the exact strategies and assays in the following units:

With a team of scientists who come from different research backgrounds, Creative Biolabs guides you all the way from project inception to high-quality results. If you have interest, please do not hesitate to contact us.

Reference

1. Lennon, F. E.; et al. The Mu opioid receptor promotes opioid and growth factor-induced proliferation, migration and Epithelial Mesenchymal Transition (EMT) in human lung cancer. PloS one. 2014, 9(3): e91577.

For Research Use Only | Not For Clinical Use