FcR Binding Assay

Fc Receptors

The Fc-gamma receptor (FcR), a member of the immunoglobulin superfamily, is divided into FcγRI (CD64), FcγRIIa (CD32a), FcγRIIb (CD32b), FcγRIIIa (CD16a) and FcγRIIIb (CD16b). Historically, these receptors have been classified based on their affinity for specific IgG subclasses and whether their binding initiates activation or transmits inhibition signals. The Fc-gamma receptor is expressed on many immune cells and binds to monomeric IgG with high affinity, thereby modulating the immune response.

Measuring the affinity of antibodies for Fc receptors has become an integral part of biotherapeutic drug development. Creative Biolabs provides off-the-shelf FcR binding assays based on our real-time kinetic analysis, flow cytometry and surface plasmon resonance (SPR) technology platforms. FcR binding assay is used to support the process development of therapeutic antibodies, such as to evaluate different clones, promote different production processes, or prepare materials for different formulations. These Fc receptor binding assays will enhance the overall understanding and demonstrate comparability in the development of therapeutic antibodies.

Binding Assay Development for Your Custom Antibody

Creative Biolabs uses a versatile label-free system with excellent sensitivity for FcγR binding analysis of various IgG antibody isotypes or Fc fusion proteins. Our team of scientists works with you to optimize the performance of FcγR binding assays and apply the experience to new antibody therapies, which can be analyzed in many different formats to meet your needs. Example assays include:

  • FcγR1/ CD64
  • FcγR2a/ CD32a R variant
  • FcγR2a/ CD32a H variant
  • FcγR2b/CD32b
  • FcγR3a/ CD16a V variant
  • FcγR3a/ CD16a F variant
  • FcγR3b/ CD16B

With our state-of-the-art technology and experienced scientists, Creative Biolabs has introduced a novel, reliable and highly flexible platform for evaluating FcγR binding properties. We can perform FcyR binding assays based on the following techniques:

  • Conventional ELISA
  • Flow cytometry
  • Surface Plasmon Resonance (SPR) / Octet Biolayer Interferometry (BLI) (recommended): real-time unlabeled full kinetic analysis, and reporting of affinity constants (KD) associated with detailed or "on" rates (Ka) and dissociation rates (Kd) or "closed" rates.

For more details, please feel free to contact us or send us a query directly.

Related Products

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FC-P006-ZP Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, ultra sensitivity (primary amine labeling) (SPR verified) [Biotinylated]
FC-P007-ZP Human FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Strep II Tag (SPR & BLI verified) [Biotinylated]
FC-P008-ZP Human FcRn / FCGRT&B2M Heterodimer Protein, His,Avi Tag (SPR verified) [Biotinylated]
FC-P009-ZP Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag (BLI verified)
FC-P010-ZP Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (BLI verified)
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FC-P012-ZP Mouse FcRn / FCGRT&B2M Heterodimer Protein, Avi Tag,His Tag&Twin-Strep Tag (BLI verified) [Biotinylated]
FC-P013-ZP Porcine FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified)
FC-P014-ZP Rat FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified)
FC-P015-ZP Rabbit FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (SPR verified)
FC-P016-ZP Rat FcRn / FCGRT&B2M Heterodimer Protein, Avitag™,His Tag&Strep II Tag (SPR verified) [Biotinylated]
FC-P017-ZP Bovine FcRn / FCGRT&B2M Heterodimer Protein, His,Avi Tag (SPR verified) [Biotinylated]
FC-P018-ZP Feline FcRn / FCGRT&B2M Heterodimer Protein, His,Avi Tag (SPR verified) [Biotinylated]
FC-P019-ZP Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified)
CD23
FC-P020-ZP Human CD23 / Fc epsilon RII Protein, His Tag
FC-P021-ZP Human CD23 / Fc epsilon RII Protein, His,Avi Tag [Biotinylated]
Fc gamma RI / CD64
FC-P022-ZP Mouse Fc gamma RI / CD64 Protein, His Tag (BLI verified)
FC-P023-ZP Cynomolgus Fc gamma RI / CD64 Protein, His,Avi Tag (BLI verified) [Biotinylated]
FC-P024-ZP Human Fc gamma RI / CD64 Protein, His Tag (MALS verified)
FC-P025-ZP Human Fc gamma RI / CD64 Protein, His,Avi Tag (MALS verified) [Biotinylated]
Fc gamma RIIA / CD32a
FC-P026-ZP Human Fc gamma RIIA / CD32a (H167) Protein, His Tag (SPR & BLI verified)
FC-P027-ZP Cynomolgus Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI & HPLC verified)
FC-P028-ZP Cynomolgus Fc gamma RIIA / CD32a Protein, His,Avi Tag (SPR & BLI verified) [Biotinylated]
FC-P029-ZP Human Fc gamma RIIA / CD32a (R167) Protein, His Tag (BLI verified)
FC-P030-ZP Human Fc gamma RIIA / CD32a (H167) Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
FC-P031-ZP Human Fc gamma RIIA / CD32a (R167) Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
FC-P032-ZP Rhesus macaque Fc gamma RIIA / CD32a Protein, His Tag (SPR & BLI verified)
Fc gamma RIIB / CD32b
FC-P033-ZP Cynomolgus Fc gamma RIIB / CD32b Protein, His Tag
FC-P034-ZP Cynomolgus Fc gamma RIIB / CD32b Protein, His,Avi Tag (SPR & BLI verified) [Biotinylated]
FC-P035-ZP Human Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified)
FC-P036-ZP Human Fc gamma RIIB / CD32b Protein, SUMO,His Tag
FC-P037-ZP Human Fc gamma RIIB / CD32b Protein, HSA,His Tag
FC-P038-ZP Human Fc gamma RIIB / CD32b Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
FC-P039-ZP Mouse Fc gamma RIIB / CD32b Protein, His Tag (SPR & BLI verified)
FC-P040-ZP Mouse Fc gamma RIIB / CD32b Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
Fc gamma RIII / CD16
FC-P041-ZP Mouse Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI verified)
FC-P042-ZP Cynomolgus Fc gamma RIII / CD16 Protein, His Tag (BLI verified)
FC-P043-ZP Cynomolgus Fc gamma RIII / CD16 Protein, His,Avi Tag (BLI verified) [Biotinylated]
FC-P044-ZP Rhesus macaque Fc gamma RIII / CD16 Protein, His Tag (SPR & BLI verified)
Fc gamma RIIIA / CD16a
FC-P045-ZP Human Fc gamma RIIIA / CD16a (V176) Protein, His Tag (SPR & BLI verified)
FC-P046-ZP Human Fc gamma RIIIA / CD16a (F176) Protein, His Tag (SPR & BLI verified)
FC-P047-ZP Human Fc gamma RIIIA / CD16a (V176) Protein, SUMO,His Tag
FC-P048-ZP Human Fc gamma RIIIA / CD16a (V176) Protein, HSA,His Tag
FC-P049-ZP Human Fc gamma RIIIA / CD16a (F176) Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
FC-P050-ZP Human Fc gamma RIIIA / CD16a (V176) Protein, Avi Tag,His Tag (SPR & BLI verified) [Biotinylated]
Fc gamma RIIIB / CD16b
FC-P051-ZP Human Fc gamma RIIIB / CD16b (NA1) Protein, His Tag (SPR & BLI verified)
FC-P052-ZP Human Fc gamma RIIIB / CD16b (NA1) Protein, His,Avi Tag (SPR & BLI verified) [Biotinylated]
FC-P053-ZP Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI verified)
FC-P054-ZP Human Fc gamma RIIIB / CD16b (NA2) Protein, His,Avi Tag (SPR & BLI verified) [Biotinylated]
Fc gamma RIV / CD16-2
FC-P055-ZP Mouse Fc gamma RIV / CD16-2 Protein, His Tag
FC-P056-ZP Mouse Fc gamma RIV / CD16-2 Protein, His,Avi Tag (BLI verified) [Biotinylated]
Fc Protein
CB-P445-K Recombinant Human Fc receptor-like protein 1 (FCRL1)
CB-P446-K Recombinant Human Fc Protein, IgG1
CB-P447-K Recombinant Human Fc Protein, IgG2
CB-P448-K Recombinant Human Fc Protein, IgG4
CB-P449-K Recombinant Mouse Fc Protein, IgG1

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