It has been demonstrated that ferroptosis plays a pivotal role in inhibiting the growth of particular tumor cells. Glutathione (GSH) is a significant antioxidant in vivo, and its targeted metabolism has also been extensively investigated for cancer therapy. Creative Biolabs offers GSH-related detection and analysis services. Moreover, we can target various steps of GSH metabolism to develop ferroptosis-mediated tumor therapy for customers, which will supply novel ideas for cancer immunotherapy.

Introduction to Glutathione in Ferroptosis

In the System Xc-/GPX4 pathway involved in ferroptosis, System Xc- is an important transporter involved in the synthesis of GSH. GPX4 plays an antioxidant role by catalyzing the conversion of GSH into GSSG, and GSH is at the center of this pathway. It can clear ROS and active nitrogen, and can also be used as an antidote for integrative detoxification. Moreover, many cancer types, including liver, lung, breast, and colon cancer, show elevated GSH levels relative to normal tissue and utilize the detoxification power of GSH to counteract the activity of antitumor drugs. As a result, the GSH system has attracted the attention of Creative Biolabs and a series of service solutions designed to reduce intracellular GSH to stop the growth of tumor cells and improve the efficiency of existing anti-cancer therapies have been developed.

Fig.1 Mechanisms of Ferroptosis.¹

Services

Creative Biolabs uses a high-throughput platform to provide mitochondrial correlation analysis and validation services in tumor cell ferroptosis. We offer a range of mitochondrial function testing systems for accurate and rapid monitoring of changes in mitochondrial function. Furthermore, our autonomous high-performance computing platform will continue to be updated and expanded to ensure efficient data processing and secure data storage.

• GSH Covalent Inhibitor Evaluation Model Services

Glutathione, which is widely found in various tissues and organs of the human body, is often used to evaluate the activity of covalent inhibitors because of its cysteine and sulfhydryl structure. We have established a GSH chemical binding evaluation model to help compound target distal cysteine accurately. At present, CBL can provide a full set of in vitro screening platforms, from protein preparation, enzymatic method development, in vitro ADME, in vivo PK, and in vivo pharmacological evaluation, which has rich experience. It has also built a high-throughput screening platform for many targets to provide research and development services for rapidly supporting drug research and the development of protein degraders. Welcome to inquiry and online communication.

• GSH Site Identification Services

Recent studies have shown that GSH is involved in the regulation of several ferroptosis-related signaling pathways. Given the potential importance of this modification in stress response and adaptation, many methods have been developed by our labs to identify and analyze glutathione-producing proteins. In general, we use biotinylated glutathione or commercial glutathione antibodies to detect glutathione protein. Biotin-labeled glutathione can also be used for the identification of glutathione proteomics in vivo.

• GSH Iron Metabolic Pathway Services

Ferroptosis is mainly regulated by the iron-based metabolism pathway, System Xc-/GPX4 pat, way, and lipid metabolism pathway. Creative Biolabs provides development and validation services for discovering acting substances and ferroptosis targets in the metabolic pathways involved in GSH.

• GSH Metabolism Balance Solutions

As a non-enzymatic antioxidant system that eliminates reactive oxygen species, the GSH metabolic balance is closely related to the occurrence of ferroptosis. Normally, we have evaluated the antioxidant effect of GSH by analyzing two ATP-dependent enzymatic reaction synthesis processes.

• Glutathione Regulatory Analysis Services In Cancers

Cancer cells generally have high levels of GSH. In Creative Biolabs, we have evaluated intracellular GSH levels and predicted the efficacy of antitumor agents against a range of GSH homeostasis indicators, such as GSH synthesizing rate-limiting enzyme activity, precursor amino acid absorption rate, and GSH/GSSG homeostasis balance.

Consumption of GSH assumes a vital role in tumor therapy. Creative Biolabs has been delving into the synthesis and metabolism analysis of GSH, as well as the identification of some key targets that regulate GSH. Moreover, we will furnish novel cancer treatment solutions for diverse mechanisms of ferroptosis-mediated GSH consumption. If you are interested in our services, please feel free to contact us.

Reference

1. Ma, Lisha, Wanqi Shao, and Weili Zhu. "Exploring the molecular mechanisms and potential therapeutic strategies of ferroptosis in ovarian cancer." Biocell 48.3 (2024): 379-386.

For Research Use Only | Not For Clinical Use