Creative Biolabs provides you with the best-tailored ferroptosis detection program, from ferroptosis protocol design, and cell culture to ferroptosis index detection. Our integrated ferroptosis solutions allow customers to experience quality and intimate service. With complete infrastructure and advanced instruments, we can provide multi-directional index detection detection services for the majority of pharmaceutical researchers and companies.

Introduction to Ferroptosis

Ferroptosis is a regulated form of cell death characterized by iron-dependent accumulation of lipid peroxidation to lethal levels. Inhibition of the cystine transport protein leads to intracellular glutathione (GSH) depletion, ultimately resulting in the inactivation of glutathione peroxidase (GPX4) and the accumulation of lipid peroxide, which can induce cell death. Ferroptosis is associated with pathological cell death in various tumor types. Ferroptosis plays a key role in inhibiting cancer cell growth, enhancing cell killing, and overcoming drug resistance. Therefore, accurate detection and analysis of ferroptosis-related metabolites hold great significance for oncology. In the past few years, Creative Biolabs has launched a series of ferroptosis-related metabolites detecting and analyzing services to better meet the needs of our worldwide customers.

Fig.1 Core Molecular Machinery and Signaling Regulation of Ferroptosis.¹

Creative Biolabs has a world-class metabolomics and oncology research services team with extensive experience in ferroptosis analysis to provide the fastest and most accurate ferroptosis detection solutions. In our labs, by utilizing proprietary oxidative fatty acids and redox metabolite passivation techniques, we can enhance the accuracy of detection data, bringing it closer to the original ecological sample conditions and more realistic. Furthermore, our team is capable of achieving a limit of quantitation as low as pg and cost-effectively integrating various indicators.

Chief Services

As a leading CRO in oncology testing, Creative Biolabs offers a wide range of sensitive bioluminescence, fluorescence, and absorbance ferroptosis testing assays. Meanwhile, we can provide ferroptosis-related signaling pathways and gene expression detection products for you to choose from. Nowadays, our ferroptosis detection solutions are mainly focused on System Xc-, GSH metabolism, GPX4 activity regulation, and ROS production analysis.

1. Oxidative Stress and Reactive Oxygen Species

Usually, Intracellular ROS levels will be detected by a DCFH-DA fluorescence probe.

2. Lipid Peroxidation

In general, the C11 BODIPY 581/591 fluorescence probe, LiperFluo fluorescence probe, and thioba were used to detect lipid peroxidation. Malondialdehyde (MDA), a product of lipid peroxidation, was detected by bituric acid reactant method (TBARS).

3. Glutathione

GSH/GSSG ratio assay will be developed to quantify glutathione levels in samples.

4. Mitochondrial

We offer mitochondria-mediated promotion and inhibition testing services. The promoting effect is mainly based on the correlation analysis of voltage-dependent anion channels and cardiolipin, while the inhibitory effect is focused on CDGSH iron-sulfur domain 1 and mitochondrial ferritin.

5. Iron Metabolism

Ferrous and/or iron ions in samples will be determined by measuring the amount of ferrous ions in living cells.

Creative Biolabs offers a wide range of efficient and innovative ferroptosis index testing solutions and reagents. Many detection systems can be stacked with apoptosis and other vitality detection reagents to sensitively compare data from plate to plate, day to day, and batch to batch. Customers only need to provide samples to be tested, and we will arrange corresponding testing services and provide professional analysis reports. If you are interested in our services, please feel free to contact us.

Reference

1. Tang, Daolin, et al. "Ferroptosis: molecular mechanisms and health implications." Cell research 31.2 (2021): 107-125.

For Research Use Only | Not For Clinical Use