Human PBMC-T Mouse Model for Immuno-Oncology Study

Background

In the evolving landscape of immuno-oncology research, humanized mouse models have revolutionized our approach to understanding the interactions between tumors and the human immune system. Creative Biolabs, a pioneer in the field, offers cutting-edge platforms that integrate peripheral blood mononuclear cells (PBMCs) into immunodeficient mice, yielding an optimized and robust model for preclinical testing. Through the PBMC-T mouse model, researchers gain invaluable insights into immune-tumor dynamics, providing a more realistic simulation of human immune responses to both tumors and therapeutic interventions.

Human PBMC-T Mouse Model for Immuno-Oncology Study

The PBMC-T mouse model is an innovative tool wherein human PBMCs are transplanted into immunodeficient mice like NSG or NOD-SCID strains. This transplantation leads to the reconstruction of human-derived T cells, establishing a human immune microenvironment within the mice. Such a setting is crucial for evaluating the efficacy, pharmacodynamics, and mechanisms of action of diverse cancer therapeutics, including checkpoint inhibitors, monoclonal antibodies, therapeutic vaccines, and cytokine-based therapies.

Our Service Contents

Creative Biolabs offers comprehensive services for creating customized humanized mouse models tailored to specific research demands. The process includes:

Selection of the Mouse Background: Immunodeficient strains such as NSG or NOD-SCID are selected to ensure successful humanization.

Transplantation of Human PBMCs and Tumor Cells: Human PBMCs are transplanted into the mice, followed by the introduction of human tumor cells or tissues.

Pretreatment and Monitoring: The mice are pre-treated and monitored throughout the humanization process to ensure stable integration of human immune cells.

Advanced techniques like flow cytometry are employed to monitor and quantify the immune cells within the mouse post-transplantation. This includes:

Quantitative and Qualitative Analysis: Regular evaluations of the type and quantity of immune cells, ensuring accurate tracking of immune cell populations.

Functional Assessments: Assessing the functional status of these cells to verify the effective reconstruction of the human immune system.

Size Tracking: Tumor growth is consistently measured to observe changes over time.

Treatment Impact Evaluation: Analyzing how different therapeutic interventions affect tumor progression.

Our platform enables thorough assessments of various immunotherapy strategies:

Immune Modulation: Evaluating the therapeutic effects of immune checkpoint inhibitors, CAR-T cells, and other modalities.

Immune Cell Analysis: Before and after treatment, immune cells are analyzed using methods like flow cytometry to determine changes influenced by the therapy.

Highlights of Our Human PBMC-T Mouse Model

Fig.1 Highlights of Our Human PBMC-T Mouse Model. (Creative Biolabs Original)

Frequently Asked Questions

Q1: What are the primary advantages of using the PBMC-T mouse model in immuno-oncology research?

A1: The PBMC-T mouse model offers a human immune microenvironment, stable reconstruction of human immune cells, extended dosing windows, and compatibility with a broad range of human tumor cells—all of which contribute to more accurate and reliable preclinical studies.

Q2: What is the significance of extending the dosing window in PBMC-T mouse models?

A2: Extending the dosing window beyond three weeks allows for more prolonged and flexible experimental timelines, enabling comprehensive studies of immune-tumor interactions and the evaluation of various therapeutic interventions.

By leveraging Creative Biolabs' innovative PBMC-T mouse models, researchers can gain a deeper understanding of immune-oncology dynamics, evaluate novel immunotherapies, and accelerate the development of effective cancer treatments.

For Research Use Only | Not For Clinical Use

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