Creative Biolabs provides iron metabolism index testing services, including serum iron, total iron-binding capacity (TIBC), transferrin saturation, unsaturated iron-bonding capacity, serum ferritin, and transferrin. We not only support routine iron metabolism testing but also can provide professional analysis solutions for ferroptosis-mediated iron metabolism pathways in tumor cells. Welcome to inquire!

Introduction to Iron Metabolism in Ferroptosis

With the deepening of ferroptosis research, a wide range of methods have been developed for iron metabolism and lipid metabolism detection. The determination of cellular iron content in iron metabolism or the ratio of Fe²⁺ to Fe³⁺ can be an important indicator for monitoring tumor cell ferroptosis. At present, for iron content detection, Creative Biolabs has established a variety of detection systems, including but not limited to the fluorescence probe method (CalcIN-AM), chemical reaction method (Prussian blue staining), and inductively coupled plasma mass spectrometry (ICP-MS). The specific detection methods of Fe²⁺ mainly focus on fluorescence probe methods, such as RhoNox-1, FerroOrange, and FRET iron Probe 1 (FIP-1).

Fig.1 The Signaling Pathways of Iron Metabolism And Ferroptosis.¹

Main Detection Systems

Creative Biolabs has devoted itself to research and independently developed a variety of iron metabolism detection systems to help ferroptosis research. We provide complete iron metabolism index testing services, involving iron ion absorption, transport, storage, and physiological functions, such as serum iron, serum total iron binding capacity, serum transferrin, serum ferritin, and serum transferrin receptor. We will develop test plans according to the requirements of product characteristics, and provide different detection services for ferroptosis-related iron metabolism index.

• ICP-MS

In Creative Biolabs, ICP-MS, as an inorganic multi-element analysis technology using inductively coupled plasma as an ion source and mass spectrometry for detection, has become the most common technology for quantifying the total iron content in the ferroptosis pathway, with the advantage of high sensitivity.

• Prussian blue staining

Prussian blue staining is a sensitive and excellent classical method for displaying trivalent iron in tissues. This method is only suitable for the determination of tightly bound or loosely bound iron ions in animal or human tissues and is not suitable for the complete detection of free iron ions.

• Fluorescent probe systems

Fluorescent probes represent a widely utilized method for detecting iron levels. Within our research facility, we commonly employ fluorescent probes containing iron-chelating groups like CALcIN-AM and Phen Green SK to track real-time fluctuations in cellular iron levels due to their ease of use, rapid detection capabilities, and versatility.

Furthermore, we have innovated a range of novel fluorescent probes tailored specifically for detecting Fe²⁺ levels including FerroOrange, Mito-FerroGreen, and RhoNox-1.Normally, the robust binding capacity exhibited by RhoNox-1 facilitates semi-quantitative analysis of Fe²⁺ within live cells or cryosections. FerroOrangea and Mito-FerroGreen serve as dedicated tools for identifying labile Fe²⁺, boasting stable fluorescence intensity along with superior permeability while maintaining low cytotoxicity. Additionally, a suite of FRET probes exemplified by FIP-1, based on intracellular peroxide reactions has been engineered to selectively monitor alterations in iron ions during ferroptosis.

Creative Biolabs' iron metabolism detection solutions offer a straightforward, rapid, and accurate approach to studying the metabolic pathways associated with ferroptosis. Additionally, a wide range of research models for iron metabolism have been generated and broadly used, and these detection systems can be seamlessly integrated. If you are interested in our services, please feel free to contact us.

Reference

1. Ge, Shihao, et al. "Iron Metabolism and Ferroptosis in Early Brain Injury after Subarachnoid Haemorrhage." Molecular Neurobiology (2024): 1-11.

For Research Use Only | Not For Clinical Use