Isolation, Culture, and Characterization Service of Neurons
As the fundamental unit of the nervous systems, neurons are responsible for receiving internal and external sensory input and implementing our complex bodily functions and behaviors. They can be classified in many ways including structure, function, connection, electrical properties, and neurotransmitter expression. Creative Biolabs offers isolation, culture, and characterization service of neurons to facilitate your microbe project's success.
Neurons
Neuron is an electrically excitable cell that communicates with other cells via specialized connections. The typical neuron generally consists of a cell body, dendrites, and a single axon (shown in Fig.1). The cell body is a compact structure and the axon and dendrites are filaments extruding from the cell body. Dendrites typically branch profusely and extend a few hundred micrometers from the cell body, and the axon leaves the soma at a swelling called the axon hillock. In most instances, neurons are generated by neural stem cells during brain development and childhood. Neurogenesis largely ceases during adulthood in most areas of the brain.
Fig.1 Anatomy of a multipolar neuron. (Wikipedia)
Classification of Neurons
TYPE
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DETAILED CONTENT
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Structural classification
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Polarity
Unipolar, Bipolar, Multipolar (Golgi I and Golgi II), Anaxonic, Pseudounipolar.
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Other
Basket cells, Betz cells, Lugaro cells, Medium spiny neurons, Purkinje cells, Pyramidal cells, Renshaw cells, Unipolar brush cells, Granule cells, Anterior horn cells, Spindle cells.
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Functional classification
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Direction
Afferent neurons, Efferent neurons, Interneurons.
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Action on other neurons
Excitatory, Inhibitory, Modulatory.
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Discharge patterns
Tonic or regular spiking, Phasic or bursting, Fast spiking.
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Neurotransmitter
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Multimodel classification
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Our Service
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Cell Isolation
Creative Biolabs offers high-quality services of rapid isolation of untouched neurons from neonatal and adult mouse brain. The untouched neurons can be purified with the neuron isolation kit. The procedure of isolation is simple and fast. It takes only one hour to acquire highly purified and viable neurons from a single cell suspension.
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Cell Culture
Cultivation of primary adult mouse neurons can be a challenging task. Creative Biolabs offers cell culture services to cultivate neurons from mouse samples. Our serum-free neuro medium improves the performance of your neuron culture.
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Cell Characterization
Creative Biolabs offers specific approaches to characterize the neurons:
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Immunofluorescence staining and microscopy
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Flow cytometry
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Single-cell sequencing
Highlights
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Modular neuronal cell workflows
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High purity of untouched isolated cells
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Serum-free and feeder layer-free system
Creative Biolabs offers a comprehensive range of solutions for isolation, culture, and characterization service of neurons. More solutions to brain-immune-gut research can be found in the unit of our Brain-Immune-Gut interaction Study. If you have any questions about your microbe project, please feel free to contact us.
For Research Use Only | Not For Clinical Use