Oligodendrocytes are the glial cells responsible for myelinating axons in the central nervous systems (CNS). They arise from precursor cells that undergo a progression of complex stages of proliferation, migration, differentiation, and maturation to produce the myelin sheath. Creative Biolabs offers isolation, culture, and characterization service of oligodendrocyte precursor cells to facilitate your microbe project success.

Oligodendrocytes

Oligodendrocytes are the glial cells responsible for producing the myelin sheath that insulates neuronal axons in the CNS. It arises from oligodendrocyte precursor cells (OPCs), also known as oligodendrocyte progenitor cells, O-2A cells, or polydendrocytes. It also can differentiate into neurons and astrocytes depending on the brain area and stage of CNS development. OPCs are generated in successive waves from several germinal regions during development. Oligodendrocytes arise mainly from parenchymal OPCs and OPCs from the subventricular zone in the adult brain. The formation of oligodendrocytes from OPCs can be divided into four complex and precisely timed stages, proliferation, migration, differentiation, and myelination. The cells present at each stage can be identified using a panel of cellular markers.

Fig.1 Diagram of the oligodendrocytic lineage progression. (Ana, 2015)

Function of OPCs

• Adult myelination
• Remyelination
  OPCs retain the ability to proliferate in adulthood and comprise 70-90% of the proliferating cell population in the mature CNS.
• Neuron-OPC interactions
  OPCs can undergo cell division while maintaining synaptic inputs from neurons.
• Immunomodulation
  OPCs may participate in both initiation and resolution of immune responses to disease or injury.

Our Service

• Cell Isolation
  Creative Biolabs offers isolation services of pre-mature oligodendrocytes from the neonatal or adult brain to meet your research needs. Isolate highly purified, viable and functional OPCs from different samples with our microbeads.
• Cell Culture
  Creative Biolabs offers the best culture conditions for oligodendrocytes. The optimized medium supports the growth and long-term survival of mouse oligodendrocytes.
• Cell Characterization
  Creative Biolabs offers specific approaches to characterize the OPCs:
• Immunocytochemistry
• Flow cytometry
• Single-cell sequencing

Highlights

• Definitive solutions for efficient and supportive workflow for oligodendrocytes.
• Comprehensive and simple solution for high yield of viable single cells.
• Cultivate in serum-free medium. It can maintain the validity of oligodendrocytes for longer.

With years of experience in Brain-Immune-Gut interaction Study, Creative Biolabs offers a comprehensive range of solutions for isolation, culture, and characterization service of oligodendrocyte precursor cells. For more details, please do not hesitate to contact us.

Reference

1. Armada-Moreira, A.; et al. Neuroinflammatory modulators of oligodendrogenesis. Neuroimmunology and Neuroinflammation. 2015, 2(4): 263

For Research Use Only | Not For Clinical Use