Kinetic Solubility Testing Service

Why Do Kinetic Solubility Testing

Heterologous expression of recombinant proteins often leads to decreased protein solubility and aggregation, and in the same way in the field of biopharmaceuticals, antibodies also aggregate after entering the body, causing reduced activity and additional immune responses.

Protein solubility and stability are affected by a variety of factors, such as pH, temperature, solvents, ionic strength, metal ion cofactors, and surfactants. In addition to this, solubility can be predicted by solvent-accessible surface area and hydrogen bond estimation algorithms. The isoelectric point (pI) of a protein is the pH of a protein with a net charge of 0 when the protein solubility is at its lowest, and the protein solubility can be improved by designing to optimize the protein pI value. Protein pI is the average value of the amino acid functional group pKa, and a lower pKa indicates that the protein is more acidic. In the case of proteins, their net charge is affected by pH and is determined by the content of all ionizable amino acids and pKa.

Fig.1 Hydrophilic amino acids. (Qing, et al., 2022) Fig.1 Schematic diagram of hydrogen bonds formed by hydrophilic amino acids with water at neutral pH.¹

Our Services

Creative Biolabs offers antibody protein solubility testing to assess the importance of an antibody or antibody fragment in biotechnology and drug development.

General solubility test

Creative Biolabs provides you with universal kinetic solubility assays during your experiments, including saturation shake flasks, potentiometric titrations, and turbidimetry.

PEG precipitation solubility test

This method is based on polyethylene glycol (PEG) titration and is used to quantitatively assess the relative solubility of proteins. The method uses approximately 200 μg of purified material, which is accurate and economical, and is suitable for high-throughput screening.
The advantages of this method are accuracy, cost-effectiveness, and suitability for high-throughput screening. It helps to assess the developability of proteins and makes them easier to implement.

Ultra-high-throughput microfluidic platform

The microfluidic platform provides more than 10,000 data points from just 20 μg of purified protein, ultimately enabling low-cost, high-throughput measurements of protein solubility.
This microfluidic platform can quantify the effect of protein solubility enhancement achieved by adding different kinds and concentrations of formulation excipients, and optimize pH to improve the solubility of protein drugs.
This microfluidic platform is highly quantitative and can be used to improve various factors that affect protein solubility and contribute to the development of new protein-based drugs.

Kinetic Solubility Testing Process

Fig.2 Kinetic solubility testing process.

Why Choose Creative Biolabs

Based on a complete technology platform and experienced scientists, Creative Biolabs has established a complete testing service process in the field of in vitro ADME detection of antibodies and proteins, which can provide you with a one-stop solution to meet your analysis needs.

Fig.3 Our advantages.

If you have any kinetic solubility testing needs, please contact us!

Reference

Qing, Rui et al. "Protein design: from the aspect of water solubility and stability." Chemical reviews. 122,18 (2022): 14085-14179.

For Research Use Only | Not For Clinical Use