Lipid peroxidation can cause serious damage to cell membranes, lipoprotein, and other lipid-containing structures, and then affect the normal function of cells. Lipid peroxidation is involved in many human diseases, such as tumors, vascular sclerosis, and aging. Creative Biolabs offers a variety of lipid peroxidation assay products and services that can be used to investigate ferroptosis mechanisms and related tumors.

Introduction to Lipid Peroxidation in Ferroptosis

Lipid peroxidation is a driving factor in the process of ferroptosis. However, unconjugated and conjugated polyunsaturated fatty acids enhance iron death in different ways, while some isoprene-derived lipids inhibit iron death despite being highly oxidizing. From this perspective, we believe that different oxidation mechanisms and products lead to differences in the effectiveness of lipids in regulating ferroptosis. Due to the important role of ferroptosis in the malignant progression and treatment of cancer, elucidation of the core mechanism of ferroptosis will lay a solid foundation for the clinical application of ferroptosis. At present, the mechanism of ferroptosis-related lipid peroxidation clearance and generation has been widely reported.

As a result, Creative Biolabs provides analysis services for the relative reactivity of various lipids to two rate-determining radical propagation mechanisms (hydrogen atom transfer (HAT) and peroxy radical addition (PRA)) and the differential product spectra. We help clients understand the role and regulatory processes of lipid peroxidation in ferroptosis, as well as the potential contribution of different oxidation products such as clipped lipids and lipid electrophiles from HAT and PRA mechanisms to ferroptosis execution.

Fig.1 Natural Product-Mediated Ferroptosis.¹

Services

In an attempt to illuminate the molecular mechanism of lipid-induced ferroptosis in tumor cells, Creative Biolabs intends to employ methodologies such as immunoprecipitation, phosphorylation site prediction, in vitro kinases, the preparation of specific phosphorylated antibodies, and lipidomics to establish the model and initiate the rapid expansion process of lipid peroxidation for inducing ferroptosis production. Subsequently, we will further validate the role of the rapidly amplified lipid peroxidation processes in ferroptosis within iron-related animal models. Ultimately, we assist our clients in screening potential molecular markers and targets for the efficacy of cancer immunotherapy.

• LPO Detection Services

Creative Biolabs can directly measure hydroperoxides in any sample containing lipid hydroperoxides using a Redox reaction with ferrous ions. Quantitative analysis of lipid peroxidation is essential to evaluate the role of oxidative damage in pathophysiological diseases. At present, we have successfully targeted and quantitatively detected nearly 30 major oxidizing lipids, including HETE, LTE4, DHA, EPA, and ARA.

• Lipid Peroxidation MDA Assay

Lipid oxidation (MDA) detection assay uses a color reaction based on the reaction of MDA and thiobarbituric acid (TBA) to produce red products, which are then quantitatively detected by colorimetry in plasma, serum, urine, animal and plant tissues, or cell lysates.

• Lipid Oxidative Degradation Products Detection Services

Lipids mainly exist in the form of glycerides and phospholipids. In our labs, we conduct the analysis evaluation from two main perspectives: the detection of lipid hydrolysates and the detection of lipid oxidation products. The former encompasses hydrolyzed products such as diglycerol, monoglycerol, and free fatty acids. The latter includes indicators like oxygen uptake, reduction of lipid substrates, formation of hydroperoxides, and formation of secondary oxidation products.

• Lipid Peroxidation And Antioxidant Activity Detection Services

We have developed a series of detection systems including but not limited to, oxygen consumption measurement system, electron spin resonance, and ultraviolet to quickly and accurately reveal the mechanism of lipid oxidation in living organisms. We will select different detection methods according to specific project purposes and conditions, or use a variety of methods to detect and analyze the evaluation of lipid peroxidation from different perspectives.

Creative Biolabs has been committed to providing research solutions for inhibiting lipid peroxidation and resisting ferroptosis in tumor cells, providing ideas for improving the therapeutic effect of cancer, and providing clues for expanding drug therapy for ferroptosis-sensitive tumors and seeking drug therapy for ferroptosis-tolerant tumors. If you are interested in our services, please feel free to contact us.

Reference

1. Pandey, Pratibha, et al. "Ferroptosis targeting natural compounds as a promising approach for developing potent liver cancer agents." Frontiers in Pharmacology 15 (2024): 1399677.

For Research Use Only | Not For Clinical Use