Creative Biolabs is dedicated to revolutionizing the discovery of treatments for human cancers. We employ state-of-the-art oncology imaging technology to address the most challenging biological inquiries in tumor lineage tracing. Our team combines business acumen with innovative thinking and problem-solving abilities. We partner with other organizations to assist in their drug discovery efforts. Partners include large pharmaceutical companies, small biotechnology companies, and universities.

Introduction to Mouse Models for Lineage Tracing

In the past few decades, Creative Biolabs has established a comprehensive imaging monitoring system for intracranial tumors, including but not limited to, CT, MRI, and PET. However, these systems are not suitable for large sample size analysis due to their complex operation and high cost. Therefore, we are dedicating ourselves to using the GFP/luciferase gene markers dual fluorescence system to construct tumor mouse models. Our mouse models have shown great advantages such as high safety, easy operation, low price, and real-time quantitative monitoring.

In our labs, U251 glioma cells with red fluorescence were inoculated into the brains of green fluorescent naked mice for dynamic in vivo fluorescence imaging. At the end of the experiment, we will extract the whole brain for continuous frozen sections, and observe the tissue structure of the transplanted tumor under fluorescence microscope and laser confocal microscope. In our tumor lineage tracing mouse models, all inoculated tumor cells were marked with fluorescence, and their sizes increased with the extension of tumor transplantation time. The processes of tumor cell migration, transplantation, and fusion with host cells can also be distinguished. Moreover, in the frozen section of transplanted tumor tissue, we can identify the origin cells that make up the tumor blood vessels as host cells (green), tumor cells (red), or tumor cells fusing with host cells (yellow).

Fig. 1 Dual-Color Tracing, Whole-Body, In Vivo Fluorescence Imaging.¹

Mouse Model Construction Platform Solutions for Lineage Tracing

Creative Biolabs' core competencies include our deep oncology expertise, our proven world-class drug development platform, and our advanced imaging platform. These will be used to drive patient impact and growth in oncology and beyond.

Recently, our dual color fluorescence lineage tracing nude mouse models have proven their significant role in tumor imaging in vivo, in studying tumor cell invasion, metastasis, and tumor angiogenesis, as well as in revealing native host cell activation in the surrounding microenvironment. Nowadays, our tumor-tracking mouse model provides a transformative platform for rapid and relevant tumor information identification and validation, significantly accelerating cancer diagnosis. Creative Biolabs' primary goal is to leverage these systems to develop novel oncology imaging techniques that are more stable, faster, and can be monitored in a real-time manner through our external collaborative work and our advanced internal imaging instruments.

Fig. 2 A Photograph of The Dual-Tracer Experimental Setup of a U251 Mouse With Tumo Exposed.²

Main Procedures

1) Obtain fresh clinical tumor samples and isolate the cells.

2) Generate a stable U251 single-cell clone expressing GFP-Luc.

3) In vitro luminescence testing of U251-GFP-Luc cells.

4) Compare the biological characteristics of U251-GFP-Luc cells with U251-Control cells.

5) Perform CCK-8 cell proliferation assay and plot the proliferation curve.

6) Analyze cell cycle distribution using logarithmic growth phase cells.

7) Conduct transwell cell invasion and migration experiments.

8) Establish a tumor lineage tracing mice model and perform in vivo imaging analysis.

9) Monitor tumor growth and assess mouse survival rate.

10) Prepare pathological sections for histological analysis, followed by statistical analysis.

Creative Biolabs is a leading CRO company that is dedicated to helping our worldwide customers shorten tumor single-cell lineage tracing analysis time. Our growth and pursuit of scientific innovation are fueled by our leading oncology imaging platform, which has a proven track record and is further enhanced by our AI-led imaging mouse model optimization and engineering capabilities. Please contact us with your project requirements, our experts are here to help.

References

1. Shen, Yuntian, et al. "Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment." Experimental and Therapeutic Medicine 10.6 (2015): 2047-2054.
2. Hamzei, Nazanin, et al. "Comparison of kinetic models for dual-tracer receptor concentration imaging in tumors." Austin journal of biomedical engineering 1.1 (2014).

For Research Use Only | Not For Clinical Use