Background

Tumor profiling involves the use of various technologies to understand and uncover the characteristics of cancer cells. Various technologies and platforms to profile genomic/transcriptomic/proteomic aberrations of cancers are aiming to guide precision treatment. The traditional molecular profiling method-polymerase chain reaction (PCR) allow for rapid, sensitive, and accurate detection of potential cancer biomarkers. The major drawback of the genome-wide application of PCR is low throughput. However, PCR is typically a good choice when the number of target regions is low and when the study aims are limited to screening or identification of known variants.

PCR-based Tumor Profiling Services

Polymerase chain reaction (PCR) has given researchers the ability to detect and identify mutations and gene expression in a variety of sample sources. Creative Biolabs provides reliable tumor gene expression profiling services to help you accelerate your research. Our PCR-based tumor profiling service is a rapid, cost-efficiency method for screening or identification of known gene expression.

With over years of experience in integrated bioanalysis and assay development services, Creative Biolabs offers a well-established platform for rapid and reliable quantitative PCR analysis. Our team can support any quantitative PCR-based (qPCR) or droplet digital PCR (ddPCR) using off-the-shelf assays or validated custom assays. Our scientists will help you settle on a PCR services workflow that fits your needs and budget. The full-service option includes RNA/DNA extraction, reverse transcription, primer/probe design, qPCR/ddPCR reaction optimization, and complete data analysis.

Workflow

One-Stop Tumor Profiling Platform

As a leading biotechnology company, Creative Biolabs has extensive experience in multiple tumor profiling technologies including IHC, FISH/CISH, PCR, and Next-Generation Sequencing（ngs）. We are dedicated to providing you with the most comprehensive and professional PCR services. Using proprietary tools, our team can custom-design PCR primers and probes to further support your experiment. Each client receives a complete study report including detailed experimental procedures, primer/probe sequences, raw data, and data analysis file with gene expression fold-change information. For more details, please feel free to contact us and we will discuss your requirements.

Frequently Asked Questions

Q1: Do I need to supply a positive and negative control?

A1: It is recommended to run a positive control and a negative template control such as RT reagent control without an RNA template added.

Q2: What sample types can be analyzed using qPCR?

A2: qPCR can be used to analyze many different biological sample types, including cultured cells, blood samples, fresh tissues, fixed-formalin paraffin-embedded (FFPE) samples, etc.

Q: What format will the data be delivered in?

A: We provide the raw data. For further analysis, you can contact us to discuss.

Published Data

Data 1: Gene expression profiling of fresh frozen and formalin-fixed, paraffin-embedded breast cancer tissues

Sample type: fresh frozen tissues and formalin-fixed, paraffin-embedded tissues

Tumor: breast cancer

Technology: RT-qPCR

Fig.1 Comparison of mean Cq of each gene in fresh frozen tissues and formalin-fixed, paraffin-embedded tissues. (Sánchez-Navarro, et al., 2010)

Data 2: Detection and quantification of circulating tumor DNA in plasma of head and neck cancer patients

Sample type: blood plasma samples

Tumor: head and neck cancer

Technology: ddPCR

Fig.2 The result of TP53 mutations in ctDNA in plasma from patients using ddPCR. (van Ginkel, et al., 2017)

References

1. Sánchez-Navarro, I.; et al. Comparison of gene expression profiling by reverse transcription quantitative PCR between fresh frozen and formalin-fixed, paraffin-embedded breast cancer tissues. Biotechniques. 2010, 48(5): 389-97.
2. van Ginkel, J.H.; et al.Droplet digital PCR for detection and quantification of circulating tumor DNA in plasma of head and neck cancer patients. BMC cancer. 2017, 17(1): 1-8.

For Research Use Only | Not For Clinical Use