Background

In situ hybridization (ISH) is a highly sensitive technique, that provides information on the location, length, and number of copies of specific genes or chromosome portions. It can detect genetic abnormalities that include gene deletions, amplifications, translocations, and fusions. At Creative Biolabs, we assist clients to determine tumor diagnosis, prognosis, and treatment selection through an ISH-based tumor profiling service.

Our ISH Capabilities

ISH is an ideal method to obtain a temporal and spatial view of the tumor's genomic profile. It can reveal associations with tumor types or subtypes, cellular morphology, and clinical behavior. Creative Biolabs has an extensive experience with ISH and is committed to providing ISH services to meet your characterization needs. Our scientists have undergone extensive training to bring you highly sensitive DNA/RNA detection in cells and tissue. Based on different automated staining platforms, our experienced team helps you reduce the complex and time-consuming process of ISH and the experimental cost.

ISH Technologies

The ISH technologies can visualize RNA/DNA targets in situ through fluorescence (FISH) and chromogenic (CISH) detection. Creative Biolabs provides FISH/CISH-based tumor profiling services utilizing both bright field and fluorescent techniques in tissues and cells, allowing you to obtain temporal and spatial information about gene expression and genetic loci. Our ISH services are suitable for the analysis of different kinds of sample types including fresh and formalin-fixed paraffin-embedded (FFPE) tissues. We provide either singleplex or duplex chromogenic detection or multiplex fluorescent detection to meet your various requirements of projects.

Key Features & Advantages

Service Workflow

The basic ISH assay includes the following steps: probe/sample preparation, target hybridization, signal amplification, and detection.

One-stop Tumor Profiling Platform

Creative Biolabs provides multi-platform tumor profiling services that perform thorough molecular testing on DNA, RNA, and proteins to identify tumor biomarkers. Our services include next-generation sequencing (NGS), polymerase chain reaction (PCR), chromogenic or fluorescence in situ hybridization (CISH or FISH), etc. Some tumor profiling tests may use one, two, or a combination of technologies to uncover cancer information. We have the ability to deal with complex situations in your projects. If you are interested in our services mentioned above, please feel free to contact us to discuss your project.

Frequently Asked Questions

Q1: Do I need to provide ISH probes?

A: Yes, you can send us your own probes. We also design and make probes as required by the customer or purchase from a company.

Q2: Can I look at more than one gene at a time?

A2: Yes, we routinely do dual ISH (two probes at the same time) and can in special circumstances do three.

Reference

1. Veselinyová, D.; et al. Selected in situ hybridization methods: principles and application. Molecules. 2021, 24;26(13): 3874.

For Research Use Only | Not For Clinical Use