Creative Biolabs offers rodent brain slice assays to help you study biochemical changes related to lesions associated with stroke, epilepsy, and Alzheimer’s disease (AD), respectively.

Rodent Brain Slice

Rodent brain slice is widely applied to study biochemical changes related to lesions associated with stroke, epilepsy, and AD, respectively. It is a favorable tool especially in the case of AD research, because of its complexity. The pathology of AD brain is characterized by the extracellular deposition of amyloid β1-42 (Aβ1-42) in amyloid plaques and the intracellular aggregation of hyperphosphorylated tau protein in neurofibrillary tangles (NFTs), all embedded in an environment of activated glial cells. There are profound differences between neonatal and mature adult brain tissue which result from different effects, such as age-dependent changes in AD.

• Adolescent brain slice

The majority of brain slice cultures are produced from mice or rats. On the 12th day of postnatal, the cytoarchitecture is established, the brain is larger and easier to manipulate and neuronal cells are likely to survive explantation. Besides, these brain slices also display high levels of plasticity and show resistance to the mechanical trauma incurred when neuronal processes are cut.

• Adult brain slice

The organotypic brain slice models of mature mice can develop the AD-like pathology, which benefits from mature adult brain morphology, metabolism and so on. There is evidence that adult organotypic brain slice cultures from 7- to 10-month-old mice independently of the transgenic modification undergo slow programmed cell death, caused by a dysfunction of the neuronal repair systems.

Workflow of Rodent Brain Slice Assay

Fig.1 Workflow of rodent brain slice assay. (Creative Biolabs)

Creative Biolabs has a whole workflow for the generation of rodent brain slice cultures. In detail, the brain tissue is firstly obtained from adolescent or adult mice. Then, the brain is cut into coronal slices by the vibratome. The slices were collected and stored. Intact slices were then cultivated in a standard medium. Finally, the slices were characterized by different methods.

• Brain tissue source:
• Cortex
• Hippocampus
• Striatum
• Thalamus
• Amygdala
• Dorsal raphe
• Periaqueductal gray matter
• Cerebellum
• Spinal cord
• Substancia nigra
• Dorsal vagal nucleus
• Ventral tegmental area

Methods of Brain Slice Characterization

Creative Biolabs has a set of professional technology platforms to help you carry out the assay. Sections from brain slice can be characterized by different methods:

• Vitality Assay: evaluate cell death and vitality
• Electrophysiology: confirm neuronal activity
• Immunohistochemistry/ TUNEL Assay: evaluate the cell composition and loss over time
• ELISA: evaluate the cellular damage or cytokine measurement
• RNA-seq Gene Expression: evaluate profile changes in gene expression

With a strong academic background in neuroscience, Creative Biolabs offers robust data to drive confident decision making throughout your Brain-Immune-Gut Based Integrative Study Research. More assays about brain slices can be found in the unit of Organotypic Brain Slice Assays. If you have any confusion about your project, please feel free to contact us.

Reference

1. Mewes, A.;et al, Organotypic brain slice cultures of adult transgenic P301S mice-a model for tauopathy studies. PLoS One. 2012, 7(9): e45017.

For Research Use Only | Not For Clinical Use