Single Cell Metabolic Analysis Services for Tumor Microenvironment Heterogeneity Research
Single cell metabolic analysis represents a powerful shift from traditional bulk metabolic assays. It enables the dissection of metabolic heterogeneity within a tumor, which is crucial for understanding treatment resistance and identifying potential targets for therapy. This technique has broad implications, not only in oncology but also in various fields of biology, such as neurobiology and immunology. At Creative Biolabs, we recognize the transformative potential of single cell metabolic analysis and have developed a suite of state-of-the-art tools and services to support researchers in this field.
Single Cell Metabolomics is the cornerstone of single cell metabolic analysis. It allows us to decipher the metabolic profiles of individual cells, revealing variations that are often masked in bulk analysis. This level of granularity is essential in the context of cancer immunotherapy, as different immune cell populations exhibit distinct metabolic characteristics.
Creative Biolabs offers comprehensive Single Cell Metabolomics services, including cutting-edge mass spectrometry techniques and high-resolution metabolite profiling. Our experts are well-versed in handling a wide range of sample types, from tumor biopsies to immune cell suspensions, ensuring accurate and reliable results.
Figure 1. Single Cell Metabolomics' Workflow. (Guo, 2021)
In the intricate ecosystem of a tumor, the spatial distribution of metabolic activity is of paramount importance. Spatial Metabolomics allows us to map the metabolic landscape within a tumor, highlighting regions of high metabolic activity, hypoxia, or nutrient deprivation. This information can guide the design of spatially targeted therapies, improving treatment outcomes.
Our spatial metabolomics services at Creative Biolabs utilize advanced imaging techniques, such as mass spectrometry imaging (MSI) and Raman spectroscopy, to provide spatially resolved metabolic information. We collaborate with researchers to generate detailed metabolic maps that contribute to a deeper understanding of the tumor microenvironment.
CyTOF is an innovative technology that combines flow cytometry with mass spectrometry, allowing for simultaneous analysis of numerous parameters at the single cell level. In the context of single cell metabolic analysis, CyTOF can provide a comprehensive view of both the metabolic and phenotypic characteristics of immune cells. This multifaceted approach is invaluable for dissecting the complex interplay between immune cells and cancer cells in the tumor microenvironment.
Creative Biolabs is at the forefront of CyTOF applications in cancer immunotherapy. We have harnessed the power of CyTOF to develop customized panels that allow researchers to probe the metabolic status of immune cells with unparalleled precision.
Figure 2 Major steps of a CyTOF procedure.
Metabolic Cytometry is a technique that integrates metabolic assays with flow cytometry, enabling high-throughput analysis of single cells. It is particularly useful in identifying metabolic subpopulations within heterogeneous cell populations, providing a crucial link between single cell metabolomics and functional characterization.
At Creative Biolabs, our Metabolic Cytometry services include a range of metabolic assays tailored to the specific needs of your research. We help you unveil the metabolic intricacies of immune cells, paving the way for targeted immunotherapies.
Dehydrogenase enzymes play a central role in cellular metabolism, and their activity can be indicative of cell viability and metabolic health. The In Situ Dehydrogenase Activity Assay is a powerful tool for assessing cellular metabolism within the tumor microenvironment. It allows for the detection of metabolic alterations in situ, providing critical information for therapy development.
Our experts at Creative Biolabs employ the In Situ Dehydrogenase Activity Assay to assess the metabolic activity of single cells within their native context. This approach offers a comprehensive view of metabolic dynamics in the tumor microenvironment, guiding the development of metabolism-targeted therapies.
Reference
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Guo, Shenghao, Cissy Zhang, and Anne Le. "The limitless applications of single-cell metabolomics." Current Opinion in Biotechnology 71 (2021): 115-122.
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