Based on our advanced toxicity assessment platforms and powerful technology, Creative Biolabs provides a complete range portfolio of assays for toxicity assessment including quantification of calcium. As an end-to-end company offering high-quality analytical development and qualification services, we have the best advantages in assisting our worldwide customers.

Introduction of Calcium

Calcium has diverse important physiological functions including muscle contraction, nerve conduction, hormone release, blood coagulation, and some metabolic processes, like bone modeling and remodeling. The balance of precipitated bone calcium and serum ionized calcium is maintained through the circulating parathyroid hormone (PTH), vitamin D, and calcitonin. PTH, secreted by the parathyroid glands, could increase the serum calcium level by mobilizing calcium from bone, decreasing bone resorption, and increasing the renal and intestinal absorption of calcium. While calcitonin, produced by the parafollicular cells of the thyroid gland, deregulates high serum calcium in the opposite.

Normally, the concentration of serum calcium is kept at a stable level. But disorders of calcium metabolism balance happen when the serum calcium concentration exceeds the minimum or maximum limits, which clinically present as hypocalcemia, hypercalcemia, bone disease, kidney disease, arteriosclerotic vascular disease, and even cancers. Therefore, serial measurements of serum calcium, a sensitive and non-invasive biomarker, could be used for the diagnosis of disease and toxicity assessment.

Fig.1 Schematic of calcium homeostasis. (Song, 2017)

Calcium Production Measurement Services at Creative Biolabs

Considering its importance, calcium detection has been researched well by scientists of Creative Biolabs. With decades of in-depth study of the toxicity assessment area, we are the one most potent professional technical developers. We will bridge the gap between your sample materials and a well-rounded assessment result of your specific treatment strategies.

The measurement of serum calcium is fraught with possible man-made errors. Despite the sampling and handling of specimens, data processing and analysis is also of great importance. The total serum calcium includes free ionic calcium, complexed calcium, and protein-bound calcium. Above 90% of the protein-bound calcium is linked to albumin with the remaining 10% bound to a variety of globulins. Therefore, there’s a need to consider the adjustment for albumin concentration to deal with abnormal calcium values and reasonable concentration changes individually.

Serum calcium could be determined by various methods, including EDTA titrimetric method, fluorimetric method, colorimetric method, atomic absorption spectrophotometry, and photonic polymer film method. Many of these procedures could be performed on semi-automatic or fully automated biochemistry analyzers, which makes it a routine service at Creative Biolabs.

Features of Quantification of Calcium Services

• Fully customized design to meet every specific demand
• Quantitative and qualitative project assessment
• Affordable price with the best quality

Normally, calcium is always quantified along with other enzymes and compounds described detailedly in our Toxicity Assessment Service, like phosphorus, albumin, and Creatine kinase. No matter which panel of biomarkers you need to test, our experienced experts at Creative Biolabs will do their best to complete your project. If you are interested in this, please feel free to contact us directly.

References

1. Song, L. Calcium and bone metabolism indices. Advances in clinical chemistry. 2017, 82: 1-46.
2. Goldstein, D. A. Serum calcium. Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd edition. 1990.

For Research Use Only | Not For Clinical Use