Monoclonal Horse Antibody Discovery Introduction

The horse is by far the mammalian species with the highest number of variants in the IgG constant region. Studies have shown that horses have 11 immunoglobulin subclasses: IgM, IgD, IgA, IgE, and 7 IgG subtypes, which are sorted according to different genotypes in the heavy chain constant region. Horses have potent immune antibodies that can effectively treat snake venom. In addition, as a large mammal, the horse is able to efficiently cultivate antibody products. Therefore, horses have an important role in the preparation and wide application of monoclonal antibodies. Based on our rich field experience and advanced monoclonal horse antibody preparation platform, Creative Biolabs provides comprehensive antibody discovery services to support monoclonal horse antibody production.

Fig 1. Horse. (Creative Biolabs Authorized)

Monoclonal Horse Antibody Discovery

The horse's immune system is unique. Instead of dying after being bitten by a snake, the horse's immune system is activated and functions rapidly by constantly producing antibodies to stop the venom from spreading and to provide effective immunization against it. In some areas, patients who have been bitten by venomous snakes are treated based on this phenomenon. Simply put, by injecting the toxin into a horse, it produces antibodies against the snake's venom. The serum of the horse is then used to prepare an antivenom to alleviate the snake's venom.

In COVID-19 research, horse antibodies show great potential. One study showed that injecting the COVID-19 echinocandin protein into horses produced neutralizing antibodies that were 20–50 times more potent against COVID-19 infection or even more. This horse-based serum therapy is not only applicable to the COVID-19 infection but is already being used for rabies, tetanus, seal bites, snake venom, and other diseases. Antibody cultivation with horses not only produces powerful neutralizing antibodies but also allows for large-scale cultivation. In the case of rabies, for example, each horse can produce 600 doses of immunoglobulin for therapeutic use.

Obtaining antibodies using phage display technology is a commonly used technique for antibody preparation. Phage display libraries can mimic natural antibody libraries. Phage display libraries do not require a tedious and time-consuming immunization process. This not only greatly improves the preparation of monoclonal antibodies, which are urgently needed for research and clinical purposes, but also provides a powerful screening capability against arbitrary antigens or even unknown antigens. The corresponding antibodies can be obtained by phage antibody library construction. What's more, different phage display libraries have different roles. Generally, commonly used high-affinity specific antibodies can be obtained from natural or semi-synthetic antibody libraries. This is an important method for most of the antibodies obtained by phage display technology, and many medium and high affinity antibody molecules have been obtained by this route. Experiments have shown that these antibodies do demonstrate excellent results. However, some experiments and studies have higher requirements for antibodies. In many cases where the specificity and affinity of the antibody are required to be high, immune antibody libraries can be constructed. Immune antibody libraries can be enriched with antigen-specific antibodies, and a large percentage of antibody libraries encode functional antibodies that recognize specific immunogens.

Creative Biolabs has a wealth of knowledge and experience in monoclonal antibody discovery. We would be happy to share with you our knowledge and experience related to monoclonal horse antibody discovery.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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