Neddylation-Specific Antibody Introduction

Neddylation is a protein post translational modification (PTM) similar to ubiquitination. Neddylation has been found to regulate a wide range of cellular activities and is relevant to the pathological processes of liver diseases, metabolic diseases, immune disorders, cancer, and many other diseases. Therefore, protein neddylation has become a popular target for drug discovery. Based on the hybridoma platform and phage display platform, Creative Biolabs provides adequate services for neddylation-specific antibody development to facilitate the progress of neddylation research.

Neddylation Modification

Neddylation is the process by which ubiquitin-like protein 8 (NEDD8) is coupled to its target protein, similar to ubiquitination. The sequences of NEDD8 and ubiquitin are reported to be 59% identical, making neddylation a PTM that resembles the process of ubiquitination. Neddylation occurs through the activation of neural precursor cell-expressed, developmentally down-regulated NEDD8 by three enzymes: an activating enzyme, a binding enzyme, and a ligase. The process is that the NEDD8 precursor is cut off at the C-terminus by a maturation enzyme, exposing Gly76, which makes the NEDD8 precursor active and functional. The NEDD8 activating enzyme catalyzes its attachment to the binding enzyme E2. It is then linked to the lysine of the target protein, catalyzed by NEDD8 ligase E3. The primary role of neddylation is to alter protein function, not degradation.

The most understood substrate for neddylation is the cullin family, which serves as the scaffolding protein for the cullin-RING ligase (CRL) complex (there are three types of ubiquitin ligases E3, one of which is the RING type). CRL is the largest family of RING types and requires multiple proteins to form a complex. Cullin is responsible for linking some of these components together. There are nine human Cullin proteins, most of which require neddylation to be active. Therefore, NEDD8 promotes ubiquitination by activating cullin. CRL controls many important biological processes, including cell cycle progression, DNA repair, signal transduction, gene transcription, embryonic development, genome integrity, and tumorigenesis. In addition, NEDD8 has multiple substrates that control a variety of biological processes. For example, neddylation can inhibit the anti-cancer protein p53, activate the oncoprotein MDM2, and regulate the activity of important molecules such as EGFR, Parkin/PINK1, HIF1α/HIF2α, caspases/IAPs/RIP1, and histone H4. Therefore, neddylation is an extremely valuable drug target. In turn, neddylation modulators are effective drug candidates. Currently, an inhibitor of the NEDD8 activating enzyme NAE, the small molecule compound MLN4924, has entered clinical trials for antitumor.

Neddylation-Specific Antibody

Neddylation plays an important role in various cellular processes, such as cell cycle progression, DNA damage response, and protein degradation, and its role in various diseases is increasingly being discovered by scientists. Especially in the treatment of tumors, NEDD8-activating enzyme inhibitors have demonstrated excellent anti-tumor effects. Several NEDD8 activase inhibitors have been approved and marketed for the treatment of tumors. Since neddylation has many activities and functions, the study of neddylation is essential. In neddylation studies, protein studies are always inevitable. Therefore, neddylation-specific antibodies occupy an important position. Neddylation antibodies can be used for various applications in neddylation protein studies, including but not limited to western blotting, immunoprecipitation assays, and immunofluorescence assays.

Creative Biolabs has a wealth of knowledge and experience about PTM specific antibody discovery. We would be happy to discuss with you our knowledge and experience in neddylation specific antibody development.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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