Cas9 Expressing Monkey Primary Carotid Artery Endothelial Cells (NHFF-1123-HX31)

• Cas9-stable cells are usually used in relevant experiments involving gene editing operations. They can stably express the CRISPR Cas9 nuclease and have become popular tools across the globe. The Cas9-expressing monkey primary carotid artery endothelial cells provided by Creative Biolabs are isolated through transduction with Cas9-lentiviral particles. These cells are cultured in a gelatin pre-coated tissue culture flask until the third passage and then are collected in vials. You can expand them for 3-5 passages at a split ratio of 1:2 under a suitable culture condition. They are easy to access for every customer as long as you have related research needs.

Detailed Product Description

• Source: Healthy cynomolgus monkeys
• Purity: ≥ 80%
• Organ/Tissue: Carotid artery
• Applications: Ideal for a wide range of CRISPR genome editing applications.

Technical Specifications

• Cell Type: Cas9 expressing cells
• Species: Cynomolgus monkey
• Cryopreservation: Cryopreserved with appropriate cryoprotectant.
• Quality Control: Rigorously control cell purity and viability.
• Cell Status: Cryopreserved

Shipping and Storage

• Shipping: Cryopreserved cells are shipped with dry ice overnight. Upon receipt, promptly transfer the frozen cells to liquid nitrogen for storage until they are ready for use. It is important to note that cells should never be stored in -20 °C or -80 °C freezer.
• Storage: Liquid nitrogen (-180°C)