Cas9 Expressing Monkey Primary Colonic Fibroblasts (NHFF-1123-HX35)

• Creative Biolabs is devoted to developing Cas9-expressing cells from various normal tissues. Our Cas9-expressing monkey primary colonic fibroblasts are isolated from cynomolgus monkeys by means of transducing Cas9-lentiviral particles. These cells are cultured in a gelatin pre-coated tissue culture flask until the third passage and then are collected in vials. You can expand them for 3-5 passages at a split ratio of 1:2 under a suitable culture condition. They can stably express the CRISPR Cas9 nuclease, which will help gene editing and further accelerate your project.

Detailed Product Description

• Source: Healthy cynomolgus monkeys
• Purity: ≥ 80%
• Organ/Tissue: Colon
• Applications: Ideal for a wide range of CRISPR genome editing applications.

Technical Specifications

• Cell Type: Cas9 expressing cells
• Species: Cynomolgus monkey
• Cryopreservation: Cryopreserved with appropriate cryoprotectant.
• Quality Control: Rigorously control cell purity and viability.
• Cell Status: Cryopreserved

Shipping and Storage

• Shipping: Cryopreserved cells are shipped with dry ice overnight. Upon receipt, promptly transfer the frozen cells to liquid nitrogen for storage until they are ready for use. It is important to note that cells should never be stored in -20 °C or -80 °C freezer.
• Storage: Liquid nitrogen (-180°C)