CCND1 Monoclonal Antibody (RNF403) (NHP-AB181)

• This product is an antibody that was generated by immunizing mouse against recombinant full length human protein. It is used only in in vitro assays.

Detailed Product Description

• Introduction: Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1.
• Conjugate: None
• Antibody Isotype: IgG2aκ
• Applications: Flow Cytometry; IF; IHC; WB

Technical Specifications

• Host Species: Mouse
• Species Reactivity: Human; Monkey; Mouse; Rat
• Immunogen: Recombinant full length human protein

Product Property

• Purification: Protein G affinity chromatography
• Format: Liquid
• Concentration: 0.2 mg/mL
• Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Storage: Store at 4⁰C.

Target Information

• Target: CCND1
• Clone: RNF403
• Clonality: Monoclonal
• Alternative Names: B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; CCND1 protein; cDNA; FLJ93625; G1/S-specific cyclin-D1; mRNA; PRAD-1; PRAD1 oncogene
• Entrez Gene ID: 595