Cynomolgus Monkey CD96 HEK293 Cell Overexpression Lysate (NHFF-0524-HX622)

• Specific protein cell overexpression lysate is routinely used as a positive control in Western blot assays. At Creative Biolabs, we constructed the Cynomolgus monkey CD96 overexpression cell lysate through the manipulation of HEK293 cells. These products with high protein purity will become helpful tools for your immunoblotting experiments.

Detailed Product Information

• Source: From healthy Cynomolgus monkey
• Host: HEK293 cells

Technical Specifications

• Calculated MW: 54.8 kDa
• Apparent MW: 109.8 kDa
• Method of Preparation: This cell overexpression lysate was meticulously collected by a series of steps including the cell homogenization in cold optimized RIPA lysis reagent with multiple protease inhibitors, cell debris centrifugation, protein concentration determination by Bradford assay, lysate boiling in 1 x SDS loading buffer containing 5% b-mercaptoethanol, and lysate lyophilization.
• Lysis Reagent: An optimized RIPA lysis reagent is used in the preparation of this product. The specific ingredients are as follows: 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, and 1mM PMSF.
• Attention before Use: Centrifuge briefly before use, then dissolve the pellet in 200μl pure water and boil for about 3 minutes.
• DNA Sequence: Met1-Met503
• Tag: His
• Quality Control: The protein quality is verified by Coomassie blue staining after protein purification.

Storage and Shipping

• Storage: Usually stored at 4°C. Once re-dissolution, please aliquot this lysate into smaller sizes and transfer them into a -80°C refrigerator.
• Shipping: Ambient temperature or ice pack.