Cynomolgus Monkey Diaphragm cDNA-Oligo-dT (NHP-cOT010)

• cDNA is a kind of DNA that is synthesized from an RNA template in a catalytic reaction. It is also produced naturally by retroviruses and then can create a provirus in the host's genome. The cDNA-oligo dT product is made of RNA extracted from freshly harvested diaphragm tissue of healthy Cynomolgus monkeys by using modern extraction technology. RNA is treated with RNase-free DNase-1 to remove residual DNA. The cDNA is primed with an oligo dT primer.

Detailed Product Description

• Organ/Tissue: Diaphragm
• Applications: Gene expression analysis by PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.

Technical Specifications

• Species: Cynomolgus monkey
• Donor Status: Healthy
• Quality Control: We confirm the integrity of each RNA sample through denatured agarose gel electrophoresis. NanoDrop analysis is employed to assess RNA purity, aiming for an A260/A280 ratio within the range of 1.9-2.1. Additionally, PCR is utilized to examine residual DNA contamination. The synthesized cDNA serves as a template for PCR amplification of the β-actin gene, and the resulting PCR product is visualized on a 1% agarose gel for analysis.
• Storage: Short term storage at -20°C; long term storage at -80°C.