Cynomolgus Monkey Lymph Nodes cDNA-Oligo-dT (NHP-cOT017)

• cDNA is a special DNA type that is prepared from a template RNA in a catalytic reaction. It is also generated naturally by retroviruses and then creates a provirus in the host's genome. The cDNA-oligo dT product provided by Creative Biolabs is made of RNA extracted from normal Cynomolgus monkeys' lymph by utilizing modern extraction technology. RNA has been removed from residual DNA to ensure product quality. The cDNA is primed with oligo dT primer.

Detailed Product Description

• Organ/Tissue: Lymph
• Applications: Gene expression analysis by PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.

Technical Specifications

• Species: Cynomolgus monkey
• Donor Status: Healthy
• Quality Control: We confirm the integrity of each RNA sample through denatured agarose gel electrophoresis. NanoDrop analysis is employed to assess RNA purity, aiming for an A260/A280 ratio within the range of 1.9-2.1. Additionally, PCR is utilized to examine residual DNA contamination. The synthesized cDNA serves as a template for PCR amplification of the β-actin gene, and the resulting PCR product is visualized on a 1% agarose gel for analysis.
• Storage: Short term storage at -20°C; long term storage at -80°C.