PCNA Monoclonal Antibody (WA-19) (NHP-AB239)

• This product is an antibody that was generated by immunizing mouse against rat PCNA protein. It is used only in in vitro assays.

Detailed Product Description

• Introduction: PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
• Conjugate: None
• Antibody Isotype: IgG2aκ
• Applications: Flow Cytometry; IF; IHC; WB

Technical Specifications

• Host Species: Mouse
• Species Reactivity: Chicken; Drosophila; Human; Monkey; Mouse; Pig; Rat; Yeast; Zebrafish
• Immunogen: Rat PCNA protein

Product Property

• Purification: Protein G affinity chromatography
• Format: Liquid
• Concentration: 0.2 mg/mL
• Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
• Storage: Store at 4⁰C. Avoid freez-thaw cycles.

Target Information

• Clone: WA-19
• Clonality: Monoclonal
• Alternative Names: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen
• Entrez Gene ID: 5111