Rhesus Monkey Semaphorin 4D HEK293 Cell Overexpression Lysate (NHFF-0524-HX819)

• The Rhesus monkey Semaphorin 4D overexpression cell lysate from Creative Biolabs is generated from the homogenate of engineered HEK293 cells and has been verified to contain a high-purity target protein. It's recommended to apply these cell lysates as a positive control of your Western blot assays.

Detailed Product Information

• Source: From healthy Rhesus monkey
• Host: HEK293 cells

Technical Specifications

• Calculated MW: 36.8 kDa
• Method of Preparation: This cell overexpression lysate was meticulously collected by a series of steps including the cell homogenization in cold optimized RIPA lysis reagent with multiple protease inhibitors, cell debris centrifugation, protein concentration determination by Bradford assay, lysate boiling in 1 x SDS loading buffer containing 5% b-mercaptoethanol, and lysate lyophilization.
• Lysis Reagent: An optimized RIPA lysis reagent is used in the preparation of this product. The specific ingredients are as follows: 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, and 1mM PMSF.
• Attention before Use: Centrifuge briefly before use, then dissolve the pellet in 200μl pure water and boil for about 3 minutes.
• DNA Sequence: Met1-Arg734
• Tag: His
• Quality Control: The protein quality is verified by Coomassie blue staining after protein purification.

Storage and Shipping

• Storage: Usually stored at 4°C. Once re-dissolution, please aliquot this lysate into smaller sizes and transfer them into a -80°C refrigerator.
• Shipping: Ambient temperature or ice pack.