Rhesus Monkey Tongue cDNA-Oligo-dT (NHP-cOT069)

• cDNA is a special type of DNA that is obtained from a template RNA in a catalytic reaction. It is also generated naturally by retroviruses and then creates a provirus in the host's genome. The cDNA-Oligo-dT product from Creative Biolabs is high-quality and contains RNA extracted from normal rhesus monkeys' tongue tissue by modern extraction technology. RNA is negative for residual DNA which ensures product quality. The cDNA is primed with Oligo-dT primer.

Detailed Product Description

• Organ/Tissue: Tongue
• Applications: Gene expression analysis by PCR, characterization of alternative splicing of mRNA, verification of genetic mutation, gene cloning and target sequencing.

Technical Specifications

• Species: Rhesus monkey
• Donor Status: Healthy
• Quality Control: We confirm the integrity of each RNA sample through denatured agarose gel electrophoresis. NanoDrop analysis is employed to assess RNA purity, aiming for an A260/A280 ratio within the range of 1.9-2.1. Additionally, PCR is utilized to examine residual DNA contamination. The synthesized cDNA serves as a template for PCR amplification of the β-actin gene, and the resulting PCR product is visualized on a 1% agarose gel for analysis.
• Storage: Short term storage at -20°C; long term storage at -80°C.