Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
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HighlightsSuccinylation is an important form of post-translational modification that mainly occurs at lysine residues. It is commonly found in organisms, particularly in many metabolic enzymes involved in central and intermediate metabolism. Succinylation regulates a variety of physiological and pathological processes in cells and plays a crucial role in numerous diseases. Therefore, the study of succinylation is of great research importance for understanding disease mechanisms and developing drugs. By using our comprehensive hybridoma platform and phage display platform, Creative Biolabs offers comprehensive research services to support the development of succinylation-specific antibodies.
Succinylation is a process in which succinyl groups are covalently bound to amino acid residues, primarily lysine residues, by succinyl donors either enzymatically or non-enzymatically, mainly in lysine residues. Lysine succinylation is widespread in eukaryotic and prokaryotic cells and regulates various pathways, including the tricarboxylic acid cycle, amino acid metabolism, and fatty acid metabolism. Succinylation modification, on the one hand, the amino acid residue gene binds a succinyl group with a larger molecular weight, resulting in a significant change in protein structure; on the other hand, the change in charge of the lysine residue from +1 to -1 causes a large change in charge, resulting in changes in the physicochemical properties and functions of the protein. Therefore, succinylation modifications have a bigger effect on protein characteristics than methylation and acetylation alterations do.
Succinylation has been found to play an important regulatory role in various diseases. In neurological diseases, succinylation establishes a connection between metabolism and protein function, offering a new avenue for drug development in this field. In inflammation, lipopolysaccharide significantly increases succinate levels, an intermediate of the mitochondrial tricarboxylic acid cycle. In turn, succinate promotes the production of IL-1β in the inflammatory response by stabilizing HIF-1α. In metabolic diseases, succinylation can modify enzymatic processes and metabolic pathways, playing a significant role in cellular metabolisms, such as the tricarboxylic acid cycle, electron transport chain, glycolysis, ketone body formation, fatty acid oxidation, and the urea cycle. In tumors, succinylation modulators can either promote or inhibit various cancers by modulating the succinylation levels of target proteins. For instance, succinylation of enolase 1 by carnitine palmitoyltransferase 1A (CPT1A) promotes BC cell proliferation, while succinylation of S100A10 promotes gastric cancer (GC) cell metastasis. The complex of KAT2A and α-ketoglutarate dehydrogenase (α-KGDH) acts as a histone H3 succinyltransferase, promoting the proliferation and development of glioma cells. Succinylation is also closely associated with liver, heart, lung, and other organ diseases.
The preparation of antigens for succinylated proteins requires special attention due to their low content and wide dynamic range in biological samples. In generally, enrichment of succinylated peptides is necessary to increase their abundance. The identification of succinylated peptides can be achieved using mass spectrometry. Additionally, the specificity of the antibody can be determined through techniques such as western blotting and immunohistochemistry. Typically, succinylation-specific antibodies are generated by immunizing animals with succinylated lysine or peptides conjugated to succinylated lysine, followed by purification using affinity chromatography.
In terms of finding PTM specific antibody, Creative Biolabs has a variety of expertise. We would be pleased to talk with you about our expertise and experience in developing succinylation-specific antibodies.
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