Antigen-Specific B Lymphocytes Sorting based Antibody Development Service

Creative Biolabs, with its leading antibody research technology and profound scientific accumulation, introduces antigen-specific B lymphocyte sorting antibody development services for infectious disease research. We specialize in providing a complete custom service from antigen design, animal immunization to specific B cell sorting, cloning, expression, and antibody function validation, dedicated to accelerating research progress and new drug development in the field of infectious diseases.

Technology Overview

Antibody development based on antigen-specific B lymphocyte sorting is an advanced biotechnology involving the selection and isolation of B lymphocytes that specifically respond to certain pathogenic antigens. This process typically starts with immunized mice or rabbits, where the antigen stimulates the immune system to produce specific antibodies. Using techniques like flow cytometry, researchers can isolate B cells from these immunized subjects that specifically react to the target antigen. Then, the antibody genes in these cells are cloned using single-cell PCR technology, expressed in vitro, and validated. These antibodies, after screening and optimization, can be used for basic research, therapeutic drug development, or diagnostic reagent development, offering high target specificity and functionality.

Advantages and Limitations of Antibody Development Based on Mouse and Rabbit Antigen-Specific B Lymphocyte Sorting:

Service Process

1. Antigen Preparation: Efficient antigen design and preparation based on infectious disease-related antigen sequences provided by clients.
2. Animal Immunization: Unique immunization strategies are used to elicit strong immune responses in specific animals, ensuring animal welfare.
3. B Cell Collection: Latest flow sorting technology is used to obtain B cells from immunized animals, with detailed documentation.
4. B Cell Screening: High-throughput technology is used to select positive B cells that bind specifically to antigens.
5. Antibody Gene Cloning: Antibody gene sequences are cloned from the selected positive B cells and constructed expression vectors.
6. Recombinant Antibody Expression: Antibodies are expressed and optimized in vitro to ensure optimal biological activity.
7. Antibody Function Validation: Expressed antibodies undergo rigorous functional validation, including affinity testing and specificity checks.

Fig.1 Schematic representation of the single-B cell sorting protocol used for antibody discovery from immunised mice.^1,2

Service Advantages

• Efficient Sorting of Antigen-Specific B Cells: Our technology ensures precise sorting of B cells highly matched with specific infectious disease antigens from thousands to tens of thousands of B cells, a key step in high-specificity antibody development.
• Rapid Development of High Affinity Antibodies: Advanced cloning technology enables us to quickly obtain antibodies with extremely high affinity, significantly shortening the R&D cycle.
• Drug-Likeness Assessment of Antibodies: Professional bioinformatics analysis and experimental validation are used to assess and optimize the drug-likeness of antibodies, laying a solid foundation for subsequent drug development.

Technical Applications

• Infectious Disease Diagnosis: Developing high-specificity diagnostic antibodies for rapid detection.
• Basic Research: Designing variously modified antibodies, such as phosphorylation, methylation, acetylation, and other post-translational modifications.
• Therapeutic Antibody Development: Providing high-affinity antibodies for constructing therapeutic antibody drugs.
• Drug Target Validation: Validating potential drug targets to provide experimental evidence for new drug development.

Creative Biolabs offers antigen-specific B lymphocyte sorting antibody development services for infectious disease research, which are not only technically advanced and comprehensive but also ensure efficient execution and high-quality results of projects. We look forward to being your trusted partner and jointly advancing the field of infectious disease research. Contact us for more service information or to request a quote.

References:

1. Starkie, DO. et al. Generation of recombinant monoclonal antibodies from immunised mice and rabbits via flow cytometry and sorting of antigen-specific IgG+ memory B cells. PLoS One. 2016, 11(3):e0152282.
2. under Open Access license CC BY 4.0, without modification.

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