Creative Biolabs specializes in using advanced phage display technology to provide highly specialized antibody and peptide development services for infectious disease research. Our services cover not only the discovery and development of antibodies and peptides but also involve multiple aspects such as antibody/peptide purification, humanization, functional validation, and more. We are committed to supporting global scientists in making breakthrough progress in the treatment and prevention of infectious diseases.
Phages are viruses that infect bacteria, characterized by a simple genetic structure and ease of genetic manipulation. This makes them ideal vectors for expressing and displaying exogenous proteins. Through genetic engineering, antibody/peptide gene fragments (such as single-chain variable fragments, scFv) can be cloned into the phage genome. These fragments can then be displayed on the surface of the phage, allowing these modified phages to bind to specific antigens. Through a series of screening processes (such as affinity selection or biotin labeling), gene fragments of antibodies/peptides with high affinity and specificity can be selected from vast phage libraries containing billions of candidates.
1. Identify and prepare target antigens in infectious diseases.
2. Obtain a large variety of antibody/peptide gene fragments through animal immunization or other methods.
3. Clone these gene fragments into phage vectors to construct phage antibody/peptide libraries.
4. Selection: Incubate the phage library with target antigens and select phage clones that bind to the antigen through multiple rounds of selection.
5. Identify positive clones and verify their binding ability with the antigen. Extract gene fragments from positive clones.
6. Expression and purification of antibody/peptide fragments: Clone the extracted gene fragments into expression vectors, express the antibodies/peptides in suitable host cells, and purify them.
7. Functional validation and characteristic analysis: Stability testing, affinity measurement, antigen binding specificity testing, etc.
8. Humanization modification of antibodies/peptides using proprietary software and technology.
Creative Biolabs has extensive experience in antibody development and offers efficient antibody/peptide development services through its phage display technology platform, meeting diverse customer needs. Our services are characterized by:
If you are interested in developing specific antibodies/peptides using phage technology, feel free to contact us. Our team is ready to communicate with you to ensure the realization of your project goals.
