B Cell-based Hydrogen/Deuterium Exchange Footprinting Assay
Fundamental Principle of the Assay
During the antigen and antibody binding process, solvent accessibility can be used to label the amino acid sequences of protein by the exchange between backbone amide hydrogen and deuterium in the solvent. The exposed protein amide hydrogen atoms are in place with deuterium, while hydrogen atoms are hided and kept unlabeled in the antibody binding location of the antigen. Changes in peptide backbone due to ligand binding and chemical labeling are easily detected and read out by MS technology and are interpreted to structure data rapidly. With advanced instruments and excellent expertise, Creative Biolabs offers hydrogen-deuterium-exchange mass spectrometry (HDX-MS) for cancer epitope analysis to clients.
Fig.1 Workflow of the bottom-up hydrogen/deuterium exchange footprinting assay.1
HDX Footprinting Assay at Creative Biolabs
The HDX-MS compares the HDX exchange rates of protein segments from the antigen alone to the peptides after an antibody bound to the antigen. Creative Biolabs is talented in eliminating the back-exchange and developing suitable enzyme digestion strategies to generate peptide-level information that best describes your antigen.
Workflow
Key Features of the Service
Our Capabilities
|
Starting Material
|
Our Deliverables
|
-
200ug of the antigen and antibody
|
-
HDX-MS data set and analysis report.
-
Determine deuterium incorporation on the sequence and 3D structure of the antigen.
|
Send Your Requirements to Us
Creative Biolabs' experienced experts offer cancer epitope mapping services using HDX-MS, providing insight into the mechanism of pathology and target therapeutic function from the protein structure view. Our HDX-MS assay is of high efficiency and cost-effective. We provide rapid data analysis and high-resolution results. If you are interested in our HDX-MS service, please contact us for a quote.
Related Services
Reference
-
Masson, G.; et al. Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments. Nature Methods. 2019, 16: 595.
For Research Use Only | Not For Clinical Use
Download our brochure